气相色谱法测葡萄酒中残留甲醇的方法研究

Study on the Method of Determination of Methanol in Wine by Gas Chromatography

建立了葡萄酒中残留甲醇的气相色谱分析方法。采用安捷伦6890N型气相色谱仪（配备氢火焰离子化检测器、安捷伦7890B型自动进样器）和HP·INNOWAX30m×0．32mm×0．25m毛细管色谱柱。氮气作为载气，流速为30mL／min，氢气为助燃气30mL／min。样品先经过蒸馏处理，再进样，以外标法定量。标准曲线线性回归方程Y=552．62x-32．096。相关系数是0．9993，在添加浓度为0．79～7．9g／L范围内，葡萄酒的加标回收率为90．1％-105．3％，相对标准偏差为0．04％。0．12％，测得葡萄酒中甲醇含量为72—230mg／L，符合我国卫生标准。此方法操作简单、灵敏，能快速和准确地测定葡萄酒中残留甲醇的含量。

Gas chromatography was established for analyzing methanol content in wine. Agilent 6890N gas chromatograph （equipped with FID, Agilent 7890B automatically sampler） and HP-INNOWAX 30 m × 0. 32 mm × 0. 25 μm capillary chromatographic column was used. Take nitrogen as the carrier gas with the flow rate of 30 mL/min, hydrogen gas as oxidant gas with the flow rate of 30 mL/min. Samples were first processed by distillation, and then into the sample, using external to fix the quota. The standard curve linear regression equation was y = 552.62x - 32. 096. The correlation coefficient was 0. 9993 in the concentration range of 0. 79 - 7.9 g/L, the recovery rate of wine was 90. 1%- 105.3% and the relative standard deviation was 0. 04% -0. 12%. The methanol content in wine was measured in the range of 72 -230 mg/L, which met the health standards in China. This method was easy and sensitive to use. It can determinethe content of methanol in wine rapidly and accurately.