基于高通量测序的沼泽山雀简化基因组微卫星位点的开发

Development of Microsatellite in Marsh Tit Parus Palustris Using Illumina Mi Seq

沼泽山雀是一种广域分布物种,目前关于该物种的微卫星位点信息较少.高通量测序技术因其获得微卫星序列更加低廉、高效而逐渐被用于微卫星筛选当中.实验选取Illumina Mi Seq高通量测序平台对沼泽山雀简化基因组进行了测序,共得到2.84M读长,共开发113 519个SLAF标签;利用MISA对所得的112 558条有效序列进行微卫星片段搜索,最终成功搜索出2 471个微卫星位点,总分布密度为870.07个/M.大量的微卫星位点为微卫星的进一步筛选提供了丰富的材料,有利于沼泽山雀后续遗传学水平方面的研究.

Marsh tit, Parus palustris, is widely found all over the world, but relatively few studies have been conducted about this specie＇ s microsatellite. To develop microsatellite, next- generation sequencing technology is thought to be the most effective. This study chooses Illumina MiSeq. From the obtained 2.84M simplified sequenced reads, 113 519 SLAFs were developed. MISA was used to search the SSRs from 112 558 effective fragments of the total SLAF. 2471 SSRs were hunted with a density of 870.07 per M simplified sequenced reads. This result is sufficient to conduct a future study to hunt SSR locis with high polymorphism used in population genetics studies.