体内抑制库普弗细胞对D-氨基半乳糖所致大鼠肝损伤的影响

The effects of inhibiting Kupffer cells on the liver function of acute liver failure rats caused by dgalactosamine

目的观察体内抑制库普弗细胞对D-氨基半乳糖(D-Gal)所致大鼠肝损伤的影响,为急性肝衰竭(acute liver failure,ALF)的防治研究提供实验依据。方法 80只SD实验大鼠随机分为造模对照组和抑制库普弗细胞组,均以D-Gal 10 mg/kg腹腔注射诱导制成ALF模型,抑制库普弗细胞组24 h后经尾静脉按10 mg/kg注入氯化钆。72 h后分别检测造模对照组和抑制库普弗细胞组大鼠的肝功能、炎性因子,1周后切取肝脏组织行光镜及流式细胞凋亡检测。结果抑制库普弗细胞组大鼠血清ALT、AST和TBIL及血清免疫学指标IL-6、IL-1β、TNF-α明显低于造模对照组(P<0.05);抑制库普弗细胞组大鼠肝脏病变及肝细胞坏死程度显著低于造模对照组;抑制库普弗细胞组存活率显著高于造模对照组(P<0.05)。结论体内抑制库普弗细胞可明显减轻D-Gal所致大鼠的肝损伤,避免或抑制库普弗细胞激活可成为ALF治疗的策略之一。

Objective To study the effects of inhibiting Kupffer cells on the liver function of acute liver failure （ALF） rats caused by dgalactosamine （D-Gal）, and then provide experimental evidence for the treatment of ALF in clinical application. Methods Eighty rats were randomly divided into 2 groups： model control group （N group） and gadolinium chloride （ GdC13 ） group （ KC group）, 10 mg/kg of D-Gal were intraperitoneally injected into both groups, 10 mg/kg of GdC13 were injected through caudal vein for KC group. Liver serologies, liver histology were examined at 72 hours after the injection of D-Gal, survival benefits were calculated at 7 days after the injection of D-Gal. Results The levels of ALT, AST and TBIL in rat serum in KC group were significantly lower than those in model control group. Inflammatory responses in KC group was also significantly lower than that in model control group. Liver histology changes and survival benefits also supported this phenomenon. Conclusion The inhibition of Kupffer cells can significantly improve liver function of ALF rats.