胚胎动力学参数与胚胎发育潜能关系的初步研究

Preliminary study of the relationship between the kinetic parameters and the developmental potential of embryo

目的探讨胚胎动力学参数与胚胎发育潜能之间的关系，为筛选发育潜能更好的胚胎提供依据。方法回顾性分析在该中心接受IVF／ICSI治疗的患者，来源为2PN的胚胎，采用time-lapse技术培养，共165枚。按照卵裂期和囊胚期常规胚胎评分标准进行分组，比较各组间胚胎动力学参数的差异。结果（1）根据受精后第3天评分分为两组：1-2级胚胎组（rt=85）和3-4级胚胎组（n=80）。3-4级胚胎组在tPNa、tPNf和t2等参数比1-2级胚胎组有所延迟，差异无统计学意义（P〉0．05）；而在第二次卵裂（t3／t4）上3-4级胚胎组要比1-2级胚胎组有所提前，差异无统计学意义（P〉0．05）。（2）根据受精后第5-6天评分分为三组：优质囊胚组（n=20）、可冻囊胚组（n=17）和劣质囊胚组（n=23）。可冻囊胚组tPNa和t2参数较优质囊胚组的发育速度稍微延迟，差异无统计学意义（P〉0．05）；劣质囊胚组比优质囊胚组的发育速度显著延迟，差异有统计学意义（P〈0．05）。在tSC、tMf／p和tSB参数方面，可冻囊胚组和劣质囊胚组较优质囊胚组的发育速度都显著延迟，三组差异有统计学意义（P〈0．05）。结论t2、tSC、tMf／p和tSB等时间参数可能与囊胚的形成具有相关性，可以用来预测胚胎的发育潜能。

Objective To explore the relationship between the kinetic parameters and the developmental po- tential of embryos, and to provide the evidences for the selection of better developmental potential embryos. Methods A retrospective analysis of 165 2PN embryos was performed in our center using time-lapse technology. The embryos were divided into different groups according to the criteria of the embryo scores and the kinetic parameters were com- pared among the groups. Results （ 1 ） The embryos were divided into two groups according D3 embryo scores ： grade 1 ~2 group（n =85） and grade 3 -4 group（n =80）. The development of the grade 3 -4 group in the parameters of tPNa, tPNf and t2 was delayed than that of the grade 1 -2 group, but without a significant difference between the two groups（P 〉0. 05）. The time of t3/t4 in the grade 3-4 group was less than that in the grade 1 -2 group, but without a significant difference（P 〉 0.05 ）. （2）The embryos were divided into three groups according D5 embryo scores： high quality blastocysts group（ n = 20 ）, frozen blastocysts group （n = 17 ） and poor quality blastocysts group （n = 23 ）. The parameters of tPNa and t2 in the frozen blastocyst group were slightly delayed than those in the high quality blastocysts group （P 〉 0. 05 ）. The time of tPNa and t2 was significantly delayed in the poor quality blastocysts group than that in the high quality blastocysts group（ P 〈 0. 05 ）. There were significant differences among the three groups in the parameters of tSC, tMf/p and tSB （ P 〈 0.05 ）. Conclusion The time parameters of t2, tSC, tMf/p and tSB may be related to the formation of the blastocyst, which can be used to predict the developmental potential of the embryos.