HSPC238对肝癌细胞中RB基因表达的影响

The effects of HSPC238 on the expression of RB in hepatoma carcinoma cells

目的观察HSPC238对肝癌细胞中RB mRNA和pRb蛋白水平的影响。方法将PcDNA3.1-HSPC238质粒和PLL3.7-HSPC238干扰载体分别转染HepG2细胞,用实时荧光定量PCR的方法检测RB mRNA的表达量;采用Western blotting法检测pRb蛋白的表达。结果和对照组相比,转染高表达HSPC238载体时,HepG2细胞中RB的mRNA水平和pRb表达量也增加;相反地,和对照组相比,转染低表达HSPC238载体时,HepG2细胞中RB的mRNA水平和pRb蛋白的表达量也减少。以上结果差异均有统计学意义(P<0.05)。结论在HepG2细胞中HSPC238对RB基因的表达存在正向调控作用。

Objective To investigate the effect of up-regulation and down-regulation of HSPC238 on the RB gene mRNA and pRb protein.Methods PcDNA3.1-HSPC238 vector and PLL3.7-HSPC238 vector was transiently transfected into HepG2 cells respectively.The level of RB mRNA was detected by real-time PCR and pRb was detected by Western blotting.Results The level of RB mRNA and pRb in up-regulation group both increased compared with control grops respectively.Conversely,the level of RB mRNA and pRb in up-regulation group both decreased compared with control grops respectively.Above results were all statistically significant（P〈0.05）.Conclusion HSPC238 could have a positive effect on the expression of the RB gene.