血浆miR-21和TGF-β_1水平在心肌梗死后与心室重构的关系

The expression levels of miR-21 and TGF-β_1 in cardiac remodelin affer myocardial infarction

目的探讨心肌梗死患者microRNA-21(miR-21)和转化生长因子(TGF)-β1的表达水平,以及调控心肌梗死后心室重构发生的作用机制。方法选取200例临床和心电图确诊为心肌梗死患者血浆和100例健康体检人群血浆,分别采用荧光定量PCR检测各组血浆miR-21和TGF-β1的表达水平。结果与健康对照组比较,心肌梗死患者血浆miR-21的表达在心肌梗死后第3天、第7天、第14天,分别为0.74±0.21 vs.2.62±0.23,vs.3.67±0.25,vs.4.13±0.27,P<0.05;心肌梗死患者血浆TGF-β1的表达在心肌梗死后第3天、第7天、第14天,分别为0.98±0.18 vs.2.35±0.24,vs.3.67±0.25,vs.4.13±0.27,P<0.05。心肌梗死患者血浆miR-21和TGF-β1随着心肌梗死后心功能变化表达上调。血浆miR-21和TGF-β1mRNA与左心室舒张末内径呈正相关(r=0.757、0.701,P<0.05)。结论心肌梗死患者血浆miR-21和TGF-β1表达上调,miR-21和TGF-β参与调控心肌梗死后的心室重构。

Objective To detect the change of exoression level of plasma microRNA-21（miR-21）and TGF-β1in cardiac remodelin affer acute myocardial infarction（AMI）of the pateins.Methods 200 pateints with AMI and 100 normal controls（age,sex matched）were enrolled.Blood samples were obtained from the normal controls and patients with AMI on the 3days,7days and 14 days.Real-time PCR was developed to detect the expression of miR-21 and TGF-β1in plasma.Results The expression of miR-21 was significantly up-regulation in the 3days,7days and 14 days in MI group than that cntrol group,0.74±0.21 vs.2.62±0.23,vs.3.67±0.25,vs.4.13±0.27up-regulation in the 3days,7days and 14 days in MI group than that cntrol group,0.98±0.18 vs.2.35±0.24,vs.3.67±0.25,vs.4.13±0.27,P〈0.05,respectively.The expression of miR-21 and TGF-β1were up-regulation with the change of cardiac function.Positive relationship between miRNA-21 expression and LVDd（r=0.757,P〈0.05）;Positive relationship between TGF-β1 mRNA expression and LVDd（r=0.701,P〈0.05）.Conclusion The expression of miR-21 and TGF-β1were up-regulation in cardiac remodelin affer AMI of the pateins,which involved in regulation in cardiac remodelin affer AMI.