流式细胞术用于鲍曼不动杆菌体外药敏试验的研究

Preliminary study on the sensitivity test of Acinetobacter baumannii in vitro by flow cytometry

目的探讨流式细胞术(FCM)方法快速检测鲍曼不动杆菌体外药敏试验的临床应用价值。方法选用碘化丙啶(PI)作为荧光染料,用FCM检测大肠埃希菌标准菌株和66株鲍曼不动杆菌临床菌株对舒氨西林、左氧氟沙星、美罗培南、头孢噻肟钠的敏感性。根据细菌培养物在不同浓度药物作用后所检测到的荧光强度来判断细菌存活率,从而推断MIC值。并与微量稀释法和VITEK仪检测结果进行比较。结果 66株鲍曼不动杆菌流式细胞荧光法抗菌药物敏感试验(FCST)结果测得舒氨西林耐药35株,左氧氟沙星耐药30株,美罗培南耐药13株,头孢噻肟钠耐药38株。采用χ2检验,分别比较与微量稀释法、VITEK仪检测判断的敏感性结果,差异均无统计学意义(P>0.05)。结论鲍曼不动杆菌流式细胞荧光法药敏试验与常规方法检测结果具有一致性,并且更快速、灵敏、客观。

Objective To investigate the clinical application significance of flow cytofluroometric（FCM）antibiotic susceptibility test for A.baumannii in vitr0.Methods The sensitivity to Ampicillin/Sulbactam,Levofloxacin,Meropenem,Cefotaxime of an Escherichia coli standard strain and 66 isolates of A.baumannii were tested with FCM by using propidium iodide as a fluorescent probe.The survival rates of the bacteria in the culture after treatment with different dosages of the antibiotics were determined according to the fluorescence strength.The MIC value of the antibiotics against the 66 strains were judged with FCM,compared with microdilution and VITEK methods.Results The antibiotic resistant strains number of sultamicillin,levofloxacin,meropenem and cefotaxime sodium were 35,30,13 and 38respectively in flow cytofluorometric antibiotic sensitivity test（FCST）of 66 strains of A.baumannii.There was no significant difference（P〈0.05）compared with the antibiotic susceptibility results by the methods of VITEK instrument and microdilution by measuring withχ2 test respectively.Conclusion The established method of FCST for A.baumannii is suitable for dectecting bacterial drug-sensitivity,which is more rapid,accurate and objective.