巨噬细胞迁移抑制因子基因多态性与结直肠癌风险研究

Association of macrophage migration inhibitory factor gene polymorphism with colorectal cancer

目的研究巨噬细胞迁移抑制因子(macrophage migration inhibitory factor,MIF)基因单核苷酸多态性(SNPs;rs755662,rs11548059,rs1049829,rs1803976)与结直肠癌发生风险的关系。方法收集共计192例结直肠癌患者(CRC)和256名正常对照者外周血样本,以聚合酶链反应和Taqman探针分析方法,检测MIF基因单核苷酸多态性;以Logistic回归模型计算不同基因型与结直肠癌发生风险的关系。结果 rs755662基因型的出现频率在CRC组和正常对照组之间差异有统计学意义(P=0.011),而在rs11548059、rs1049829和rs1803976位点则差异无统计学意义(P=0.660、P=0.700和P=0.959)。此外,rs755662还分别与早期发病(年龄≤50岁,P=0.026)、分期(Ⅳ期,P=0.038)以及分化(P=0.040)有关。与正常对照组比,rs755662与Ⅲ期和Ⅳ期显著相关(P值分别为0.034和0.003)。结论 MIF基因5′-UTR区域rs755662(G/C)单核苷酸多态性与结直肠癌的易感性、患者发病年龄和分期有关。

Objective To study the single nucleotide polymorphisms（SNPs;rs755662,rs11548059,rs1049829,rs1803976）of macrophage migration inhibitory factor（MIF）gene with the incidence of colorectal cancer.Methods The peripheral blood specimens of 192 patients with colorectal cancer（CRC）and 256 normal controls were collected.PCR and Taqman probe analysis methods were used to detect the genotypes of MIFgene.Logistic regression model was used to calculate the relationship between genotypes and colon cancer risk.Results The frequency of occurrence of genotype rs755662 was significantly different（P=0.011）between CRC and normal control group,while at the loci rs11548059,rs1049829 and rs1803976there were no statistically significant differences（P=0.660,P=0.700,and P=0.959）.Moreover,rs755662 was also associated with early onset（age〈 50years,P=0.026）,and staging（Ⅳ period,P=0.038）and differentiation（P=0.040）.Compared with the normal control group,rs755662 was significantly associated with theⅢ and Ⅳ stages（P=0.034 and 0.003,respectively）.Conclusion The single nucleotide polymorphism of MIFgene 5′-UTR region rs755662（G/C）is associated with colorectal cancer susceptibility,onset age and tumor stage.