乳糖酶活性检测方法研究进展

Advances in research on method for assay of lactase activity

乳糖酶是人类肠道分解利用乳糖必需的一种双糖酶,乳糖酶在食品医药、分析、环保及遗传学等相关学科都具有重要应用,引起了国内外的广泛关注,其活性的检测方法对乳糖酶制剂的研究及乳糖酶缺乏症的诊疗具有重要的作用。乳糖酶活性定量检测方法主要有:小肠黏膜活检法、ONPG试验法、稳定同位素法、高效液相色谱法及粪便还原糖测定法;定性检测方法主要有呼氢试验法、乳糖耐受试验法及尿半乳糖比色法等。本研究通过对乳糖酶活性检测方法的研究进展进行综述,为乳糖酶检测方法的改进及新方法的建立提供参考。

Lactase is a type of disaccharidase in human small intestine which is vital to the decomposition of lactose.Lactase is widely used in food industry,medicines,analytical chemistry,environmental protection and genetics etc.It has attracted wide attention both at home and abroad.The method for assay of lactase is important to the research of lactase enzyme and the diagnosis of lactase deficiency.Methods for quantitative determination of lactase include biopsy of small intestinal mucosa,ONPG test,stable isotope technique,high performance liquid chromatography and detection of fecal reducing sugar and so on,while Methods for qualitative determination include H2 breath test,lactose tolerance test,urine galactose test and so on.This article reviewed the progress in research on the methods for assay of lactase in order to provide reference for the improvement of the detection.