人胃癌微环境来源间充质干细胞促进胃癌细胞BGC-823的体外增殖、迁移和促血管生成能力

Mesenchymal stem cells derived from gastric cancer microenvironment promote the proliferation,migration and angiogenesis of gastric cancer BGC-823 cells in vitro

目的探讨人胃癌微环境来源间充质干细胞(GC-MSCs)能否促进胃癌细胞增殖、迁移及其体外促血管生成能力。方法采用MTT增殖试验和细胞克隆形成试验观察GC-MSCs细胞对胃癌细胞系BGC-823生长的影响;Transwell迁移试验检测GC-MSCs细胞对BGC-823细胞迁移能力的影响;利用管形成试验观察并分析GC-MSCs细胞对BGC-823细胞促血管生成能力的影响;RT-PCR检测GC-MSCs细胞作用BGC-823细胞后其促血管生成相关基因(VEGF、MIP-2和TGF-β1基因)的表达情况。结果 MTT增殖试验和克隆形成试验结果表明,GC-MSCs来源的细胞培养上清可以明显促进胃癌细胞BGC-823的体外生长(P<0.05);Transwell迁移试验结果表明,GC-MSCs来源细胞培养上清对BGC-823的迁移能力具有显著促进作用(P<0.01);RT-PCR检测结果显示,GC-MSCs来源的细胞培养上清可明显上调促血管生成相关因子(VEGF、MIP-2和TGF-β1基因)在BGC-823细胞中的表达水平;管形成试验结果显示,BGC-823和GC-MSCs细胞共培养上清与BGC-823、GC-MSCs单独培养组相比具有更强的促血管生成能力。结论 GC-MSCs可通过旁分泌的方式促进胃癌细胞增殖、迁移及其促血管生成能力,进而诱导胃癌的恶性转化。

Objective To investigate the effects of mesenchymal stem cells derived from gastric cancer microenvironment（ GC-MSCs）on the proliferation,migration and angiogenesis of gastric cancer cells. Methods The effect of GC-MSCs on the proliferation of gastric cancer BGC-823 cells was determined by MTT assay and colony formation assay. The effects of GC-MSCs on the migration and angiogenesis of BGC-823 cells were detected with Transwell migration assay and tube formation assay,respectively. The expression levels of angiogenesis-related genes such as VEGF,MIP-2 and TGF-β1 were analyzed by RT-PCR. Results The supernatant derived from GC-MSCs culture promoted the proliferation,migration and angiogenesis of BGC-823 cells,and up-regulated the expression levels of VEGF,MIP-2 and TGF-β1 genes obviously（ P〈0. 05）. Conclusion GC-MSCs may promote the proliferation,migration and angiogenesis of gastric cancer cells by paracrine and then induce the malignant transformation of gastric cancer.