枯草芽孢杆菌产芽孢发酵培养基的优化研究

Study on Optimization of Spore Production in Fermentation Medium about Bacillus subtilis B53

在三角瓶培养条件下,采用单因素实验对枯草芽孢杆菌B53发酵培养基碳源、氮源进行了优化.在此基础上进行了正交实验,确定了菌株B53最佳的产孢发酵培养基为葡萄糖25g/L、大豆蛋白胨14g/L、玉米浆17g/L、NaCl 3g/L,MgSO40.2g/L.在最优条件下发酵培养,芽孢浓度为9.6×10^9个/mL.

The carbon and nitrogen sources were optimized by single factor experiment under the condition of triangular flask culture.On the basis of the orthogonal experiment, the optimum fermentation medium of strain B53 was determined （glucose 25g/ L, soybean peptone 14g/L, corn syrup 17g/L, NaCl 3g/L, MgSO4 0.2g/L） .Under the optimum conditions, the spore concentration was 9.6×10^9/mL.