毛细管电泳技术在氨基酸分离教学实验中的应用

Study on separation of amino acids with capillary electrophoresis and its application in teaching experiment

在本科生物化学实验课程中,原氨基酸分离实验采用传统的纸层析法分离,该方法存在耗时长、试剂毒性大、准确性差、斑点不清晰等缺点。针对传统纸层析法的不足,应用毛细管电泳对氨基酸进行分离,并对影响分离效果的条件进行了优化。改进后的实验课程,在更安全有效分离氨基酸的基础上,极大地提高了学生实验的主动性和积极性。

In traditional biochemical experiments,amino acids were separated with paper chromatography,which possesses several disadvantages such as time-consuming,poisonous reagents,low sensitivity and so on.In view of the above shortcomings,the capillary zone electrophoresis method was applied for separation of amino acids.Separation conditions were also systematically optimized.The whole separation procedure was more safely and effectively with capillary electrophoresis.In addition,students＇ enthusiasm and initiative were also enhanced.