摘要
在本科生物化学实验课程中,原氨基酸分离实验采用传统的纸层析法分离,该方法存在耗时长、试剂毒性大、准确性差、斑点不清晰等缺点。针对传统纸层析法的不足,应用毛细管电泳对氨基酸进行分离,并对影响分离效果的条件进行了优化。改进后的实验课程,在更安全有效分离氨基酸的基础上,极大地提高了学生实验的主动性和积极性。
In traditional biochemical experiments,amino acids were separated with paper chromatography,which possesses several disadvantages such as time-consuming,poisonous reagents,low sensitivity and so on.In view of the above shortcomings,the capillary zone electrophoresis method was applied for separation of amino acids.Separation conditions were also systematically optimized.The whole separation procedure was more safely and effectively with capillary electrophoresis.In addition,students' enthusiasm and initiative were also enhanced.
出处
《实验技术与管理》
CAS
北大核心
2016年第9期163-165,196,共4页
Experimental Technology and Management
基金
陕西省教改项目"生物工程专业贯通式创新人才培养实验区建设"
西安交通大学教改项目"生物工程综合开放实验课程建设与实践"
关键词
毛细管电泳
氨基酸
生物化学实验
capillary electrophoresis
amino acid
biochemical experiments