刺络泻血对原发性肝癌大鼠血清AFP、SF的影响

Research on the Influence of Pricking Blood on Colleterals Therapy on Serum AFP and SF in the Rat Model of Primary Liver Cancer

目的:观察刺络泻血对原发性肝癌(Hepatocellular Carcinoma,HCC)模型大鼠甲胎蛋白(Alpha-Fetoprotein,AFP)、铁蛋白(Serum Ferritsn,SF)及肝功能相关指标的影响,探究刺络泻血对HCC的预防作用及相关机制。方法:将40只健康Wistar大鼠随机分为正常对照组(10只)、模型组(15只)、刺血预防组(15只)。除正常对照组外,其余两组均采用二乙基亚硝胺(Diethylnitrosamine,DEN)腹腔注射建立HCC模型,刺血预防组在造模开始同时取肝经浅表络脉刺血,0.5 mL/次,2次/周,至13周。第13周末,腹主动脉取血5 mL,采用ELESA法测定3组大鼠血清肝功能丙氨酸氨基转移酶(Alamine Aminotransferase,ALT)、碱性磷酸酶(Alkaline Phosphatase,ALP)、谷氨酰转肽酶(Gamma Glutamyl Transpeptidase,GGT)和肿瘤标志物AFP、SF含量。结果:与正常对照组比较,模型组大鼠血清ALT、ALP、GGT、AFP、SF含量均明显升高(P<0.05),差异具有统计学意义。与模型组比较,刺血预防组大鼠血清ALT、ALP、GGT、AFP、SF含量均降低(P<0.05),差异有统计学意义。结论:刺络泻血疗法对HCC的发展有一定程度的抑制作用,其作用机制可能与降低血清肝功能指标,保护肝脏,下调肿瘤标志物AFP、SF水平有关。

Objective：To observe the effect of pricking blood on colleterals therapy on AFP and SF in rats model of primary liver cancer （HCC）,and explore its preventive effect and related mechanism.Methods：Forty healthy Wistar rats were randomly divided into control group （n =10）,model group （n =15）and pricking blood group （n =15）.In addition to the control group,the other two groups were injected with DEN （Diethylnitrosamine）intraperitoneally to establish liver cancer model.Rats in pricking blood group was performed of blood letting on the superficial veins of hind leg along the liver meridian,0.5 mL each time and 2 times per week,till the eighteenth week.At the end of the week,taking 5 mL blood from the abdominal aorta and using ELESA method to de-termine ALT （Alamine Amino Transferase）,ALP （Alkaline Phosphatase）,GGT （Gamma Glutamyl Transpeptidase）,AFP and SF levels in serum.Results：Compared with the control group,the serum levels of ALT,ALP,GGT,AFP and SF in model group were significantly increased,and the difference was statistically significant （P 〈0.05）.Compared with the model group,the serum levels of ALT,ALP,GGT,AFP and SF in the prevention group were lower,and the difference was statistically significant （P 〈0.05）. Conclusion：Blood pricking on colleterals therapy has inhibitory effect on HCC,which might due to the fact that it can decrease the liver serum levels and tumor signals AFP and SF.