川芎赤芍干预脑缺血再灌注大鼠血管新生的实验研究

Study of Sichuan Lovage Rhizome and Red Peony Root on Intervening Angiogenesis of Rats with Cerebral Ischemia-reperfusion

目的:观察川芎赤芍对大鼠脑梗死再灌注后血管生成素-1(Ang-1)、缺氧诱导因子-1a(HIF-1a)的影响。方法:线栓法制备大鼠大脑中动脉缺血再灌注模型,将SD大鼠随机分为空白组、模型组、川芎赤芍低、高剂量组、银杏叶片组。ELISA法测定大鼠血清Ang-1,实时荧光定量PCR方法检测缺血脑组织HIF-1a mRNA表达。结果:大鼠血清Ang-1测定:给药3d、7 d、14 d模型组与空白组相比升高,具有统计学意义(P<0.05);给药3 d、7 d、14 d模型组与川芎赤芍低剂量组、高剂量组、银杏叶组相比水平低,具有统计学意义(P<0.05);实时荧光定量PCR检测HIF-1a mRNA:给药3 d、7 d、14 d模型组与空白组相比表达升高,具有统计学意义(P<0.05);给药3 d、7 d、14 d模型组与川芎赤芍低剂量组、高剂量组、银杏叶组相比表达水平低,具有统计学意义(P<0.05)。结论:川芎赤芍可升高缺血再灌注大鼠血清中Ang-1的水平及缺血脑组织中HIF-1a mRNA的表达,提示川芎赤芍治疗脑梗死机制可能与促进血管新生有关。

Objective：To observe the effect of Sichuan Lovage Rhizome and Red Peony Root on angiogenin-1 （Ang-1）,ischemia induced factor-1 a （HIF-1 a）on rats with cerebral ischemia-reperfusion.Methods：The model of rat with middle cerebral artery is-chemia-reperfusion was established with suture-occluded method.SD rats were randomly divided into control group,model group and Sichuan Lovage Rhizome and Red Peony Root low and high dose groups,Ginkgo group.Ang-1 in rat serum was tested with ELISA method.HIF-1a mRNA expression in ischemia brain tissue was detected with real-time fluorescent quantitative polymerase chain reaction （rt-PCR）.Results：Ang-1 in rat serum in model groups increased on days 3,7 and 14 compared with the control group （P 〈0.05）.HIF-1a expression with Rt-PCR increased in model groups on day 3,7 and 14 compared with control group （P〈0.05）.HIF-1a expression in model group on day 3,7 and 14 was lower than Sichuan Lovage Rhizome and Red Peony Root low and high dose groups,Ginkgo group （P 〈0.05）.Conclusion：Sichuan Lovage Rhizome and Red Peony Root could enhance Ang-1 in rats with cerebral ischemia-reperfusion and HIF-1a mRNA expression in ischemic brain tissue,suggesting that the mechanism of Sichuan Lovage Rhizome and Red Peony Root on treating cerebral ischemia may be related with promoting angiogenesis.