不同纯化方法对丹参水提取液中丹酚酸B的含量影响

Effects of Different Purification Methods on Content of Salvianolic Acid B in Water Extract from Salvia Miltiorrhiza Bge

目的:考察不同纯化方法对丹参水提液中丹酚酸B含量的影响,以优选出丹参水提取液的最佳纯化方法。方法:以丹酚酸B含量和浸膏得率为考察指标,对乙醇沉淀法、高速离心法和大孔树脂吸附法3种纯化方法进行了比较。结果:大孔树脂吸附法处理后所得到的丹酚酸B的含量最高,浸膏得率最低;乙醇沉淀法处理后丹酚酸B的含量最低,浸膏得率居中;高速离心法处理后丹酚酸B的含量居中,但浸膏得率最高。结论:大孔树脂吸附法为丹参水提液的最佳纯化方法。

Objective：To explore the best purification method by observing effects of different purification ways on the extractive rate of salvianolic acid B in Salvia Miltiorrhiza Bge water-extraction.Methods：Salvianolic acid B content and dry extract yield rate were used as indexes for assessing the best purification method among ethanol precipitation,high speed centrifugation and macroporous resin absorption.Results：Among all three methods,salvianolic acid B content by macroporous absorption resin was the highest,with dry extract yield rate being the lowest;salvianolic acid B content by ethanol precipitation was the lowest,with dry extract yield rate being the medium level;and salvianolic acid B content by high speed centrifugation was at the medium level, with dry extract yield rate being the highest.Conclusion：Water extraction of Salvia miltiorrhiza Bge by macroporous absorption resin is considered as the best purification method.