蜜炙酸枣仁中斯皮诺素含量测定

Determination of Spinosin in Honey-fried Semen Zizyphi Spinosae

目的：以高效液相色谱法测定蜜炙酸枣仁中斯皮诺素的含量。方法：采用反相高效液相色谱法,以Wonda Cract ODS-2（250 mm×4.6 mm,5μm）为色谱柱,乙腈-水为流动相,检测波长335 nm,流速1 mL/min,柱温30℃。结果：斯皮诺素的线性范围0.005~0.3 mg/mL,r=0.9965,平均回收率为99.74%,RSD=1.46%。结论：该方法准确、重现性好,可为蜜炙酸枣仁的炮制工艺研究及质量控制提供检测标准。

Objective：To determine the content of Spinosin in Honey-fried Semen Zizyphi Spinosae（Mizhi Suanzaoren）by HPLC （High Performance Liquid Chromatography）.Methods：The experiment was conducted in the condition of WondaCract ODS-2（250 mm ×4.6 mm,5μm）,mobile phase isacetonitrile-water,detection wavelength 335nm,and flow velocity 1 mL/min,column tempera-ture 30 ℃,using RP-HPLC.Results：The linearity range of Spinosin is 0.005 ~0.3 mg/mL,r =0.9965,average recovery rate is 99.74%,RSD =1.46%.Conclusion：This method is accurate with great reproducibility.It can offer testing standards for process-ing study and quality control of Honey-fried Semen Zizyphi Spinosae.