摘要
目的:研究雪莲十味片总黄酮的超声提取工艺。方法:以总黄酮含量为考察指标,采用正交实验筛选出雪莲十味片提取总黄酮最佳路线,以芦丁为对照品,紫外可见分光光度法测定复方片总黄酮的含量。结果:雪莲十味片总黄酮提取最佳方法为75%的乙醇溶液,在55℃下,超声提取50 min。平均提取量12.27 mg/g。结论:优选出的最佳工艺简单易行,能够大大提高雪莲十味片的总黄酮提取率。
Objective：To study the ultrasonic extraction process of total flavonoids in Xuelian Shiwei tablet.Methods：Taking the total content of flavonoids as the detect index,and the orthogonal experiment design was adopted to investigate the best route for ex-tracting the total flavonoids from Xuelian Shiwei tablets.Taking rutin as reference,and ultraviolet spectrophotometry was applied to determine the content of flavornoids.Results：The best method of extraction is 75% ethanol as solvent,at 55 ℃,for 50 mins under ultrasonic.The average extraction volume was 1.287 mg/g.Conclusion：This method is easy to operate,and can enhance the ex-traction rate in a large deal.
作者
苏苗
红梅
阿拉腾敖日格勒
包勒朝鲁
Su Miao Hong Mei Alatan Aorigele Bao Lechaolu(Mongolian Medicine Research Institute of Inner Mongolia Medical University, Hohhot 010110, China)
出处
《世界中医药》
CAS
2016年第6期1088-1090,共3页
World Chinese Medicine
基金
内蒙古自治区蒙中药重点实验室提升计划项目资助课题(编号:20090902)
关键词
雪莲十味片
总黄酮
正交实验
紫外分光光度法
Xuelian Shiwei tablets
Total flavonoids
Orthogonal experiment
Ultraviolet spectrophotometry
