摘要
目的:研究雪莲十味片总黄酮的超声提取工艺。方法:以总黄酮含量为考察指标,采用正交实验筛选出雪莲十味片提取总黄酮最佳路线,以芦丁为对照品,紫外可见分光光度法测定复方片总黄酮的含量。结果:雪莲十味片总黄酮提取最佳方法为75%的乙醇溶液,在55℃下,超声提取50 min。平均提取量12.27 mg/g。结论:优选出的最佳工艺简单易行,能够大大提高雪莲十味片的总黄酮提取率。
Objective:To study the ultrasonic extraction process of total flavonoids in Xuelian Shiwei tablet.Methods:Taking the total content of flavonoids as the detect index,and the orthogonal experiment design was adopted to investigate the best route for ex-tracting the total flavonoids from Xuelian Shiwei tablets.Taking rutin as reference,and ultraviolet spectrophotometry was applied to determine the content of flavornoids.Results:The best method of extraction is 75% ethanol as solvent,at 55 ℃,for 50 mins under ultrasonic.The average extraction volume was 1.287 mg/g.Conclusion:This method is easy to operate,and can enhance the ex-traction rate in a large deal.
作者
苏苗
红梅
阿拉腾敖日格勒
包勒朝鲁
Su Miao Hong Mei Alatan Aorigele Bao Lechaolu(Mongolian Medicine Research Institute of Inner Mongolia Medical University, Hohhot 010110, China)
出处
《世界中医药》
CAS
2016年第6期1088-1090,共3页
World Chinese Medicine
基金
内蒙古自治区蒙中药重点实验室提升计划项目资助课题(编号:20090902)
关键词
雪莲十味片
总黄酮
正交实验
紫外分光光度法
Xuelian Shiwei tablets
Total flavonoids
Orthogonal experiment
Ultraviolet spectrophotometry