产细菌素戊糖乳杆菌B8发酵动力学模型的建立

Establishment of fermentation kinetic models for bacteriocin-producing Lactobacillus pentosus B8

从四川香肠中选育出一株具有产细菌素能力的戊糖乳杆菌B8，在10L发酵罐内对其进行分批发酵动力学研究，建立菌体生长、产物合成、底物消耗及pH随时间变化的动力学模型，确定模型参数，得到其菌体生长动力学模型dX/dt=0.5669（1-X/1.908）、细菌素生产动力学模型dX/dt=75.25 dX/dt＋122.94X、葡萄糖消耗动力学模型-dX/dt=5.843 dX/dt＋0.7998X。pH值变化的数学模型y=2.2228/1＋（x＋5.0917） 2.0938 ＋5.9928。结果表明，模型预测值与实验值吻合较好，为工业放大生产细菌素提供了理论依据和方法。

Lactobacillus pentosus B8 with bacteriocin production was screened from Sichuan sausage, and batch fermentation kinetic study was investigated in 10 L fermentor. The kinetic models of cell growth, bacteriocin production, substrate consumption and pH with time were established,model parameters were determined. The cell growth model was dX/dt=0.5669（1-X/1.908）,the bacteriocin production model was dX/dt=75.25 dX/dt＋ 122.94X, the substrate consumption model was - dX/dt=5.843 dX/dt＋0.7998X, and pH model was y =2.2228/1＋（x/5.0917）＋5.9928.The results obtained from model were in agreement with experiments data, indicating that the models had good applicability.