高效液相色谱法测定车厘子中果糖、葡萄糖及蔗糖含量

Determination of fructose, glucose and sucrose contents in cherry by HPLC

采用高效液相色谱法(HPLC)测定车厘子中果糖、葡萄糖及蔗糖的含量。测定方法:Shodex SUGAR系列KS-801(钠型)色谱柱(8 mm×300 mm,6μm)、流动相为超纯水,流速0.8 m L/min,柱温80℃,示差折光检测器(RID)温度30℃,进样量20μL,外标法定量。该法对果糖、葡萄糖和蔗糖测定的平均回收率分别为99.96%、100.30%、99.26%,相对标准偏差(RSD)分别为1.54%、0.49%、0.48%,检出限分别为3.50μg/m L、4.88μg/m L、7.50μg/m L,果糖、葡萄糖和蔗糖的标准曲线相关系数均在0.999以上(n=5)。该法可快速准确的对果糖、葡萄糖和蔗糖定性、定量分析。结果表明,车厘子果肉中果糖、葡萄糖、蔗糖的含量分别为5.69%、5.83%、0.24%。

HPLC was used to determine the content of fructose, glucose and sucrose in cheery. The operating conditions were confirmed as follows： Shodex SUGAR series KS-801 （Na＋） column （8 mm×300 mm, 6 μm）, which was held at a constant temperature of 80 ℃, using a mobile phase composed of pure water at a flow rate of 0.8 ml/min. The refractive index detector temperature was set as 30℃ and injection volume 20 μL The average recovery of fructose, glucose and sucrose determination rate were 99.96%, 100.30%, and 99.26%, respectively, the relative standard deviation （RSD） were 1.54%, 0.49%, and 0.48%, respectively, and the detection limits were 3.50 μg/ml, 4.88 μg/ml, 7.50 μg/ml, respectively. The correlation coefficients of sucrose, glucose and fructose were all above 0.999 （n=5）. The method can be used for qualitative and quantitative analysis of fructose, glucose and sucrose quickly and accurately. The results showed that the percentage composition of fructose, glucose and sucrose in cheery were 5.69%, 5.83%, 0.24%.