银杏叶提取物不同给药方式对骨质疏松大鼠成骨的影响

Effect of ginkgo biloba extract on osteogenesis by different administration routes in osteoporosis rats

目的探讨银杏叶提取物(Ginkgo Biloba Extract,GBE)不同体内给药方式对骨质疏松(Osteoporosis,OP)大鼠成骨的影响。方法构建去势大鼠骨质疏松模型(osteoporosis in ovariectomized rats,OVX)64只和糖皮质激素性骨质疏松大鼠模型(Glucococticoid-induced osteoporosis in rats,GIOP)64只。并将两组骨质疏松大鼠用随机数字表法分为3组不同浓度灌胃给药(100 mg/(kg·d)、200 mg(kg·d)、400 mg(kg·d)和3组注射给药(3.5 mg/(kg·d)、4.0 mg/(kg·d)、4.5 mg/(kg·d)),设立模型对照组2组,空白对照组1组(每组8只)。采用ELISA染色法检测血清骨钙素(OC)、抗酒石酸酸性磷酸酶(TRAP),观察股骨横切HE染色病理改变。结果与对照组相比,两组大鼠构模2个月后,OC、TRAP水平检测,大鼠股骨远端横断观察,符合骨质疏松改变。两组骨质疏松模型通过不同方式和浓度的给药后,与不给药对照组相比,OC水平上升(P<0.01),TRAP水平下降(P<0.05)。组织学观察可见,两组大鼠给药后均有不同程度新生骨小梁形成,骨小梁排列整齐,有不同程度的变粗,数量增多。结论 GBE灌胃给药与腹腔注射给药对骨质疏松大鼠的成骨均有促进作用,促进成骨细胞分泌OC,抑制破骨细胞的分泌TRAP,可以促进骨小梁的形成,增强骨的致密度,作用效果与给药方式和剂量有直接关系。

Objective To explore the effect of ginkgo biloba extract（GBE） on osteogenesis in osteoporosis（OP） rats by different administration routes.Methods Two groups of osteoporosis models were developed by ovariectomy（OVX） and glucocorticoid^nduced（GIOP） method（64 rats in each group）.The rats were divided by random number table into 3 concentration groups（100 mg/kg/d,200 mg/kg/d,and 400 mg/kg/d） by intragastric administration,and 3 concentration groups by injection（3.5 mg/kg/d,4.0 mg/kg/d,and 4.5 mg/kg/d）.Two model control groups and a blank control group（8 rats in each group）were established.Serum levels of OC and TRAP were detected using ELISA method.Bone slices were HE-stained and observed.Results Compared with the control groups,the osteoporosis rat model was constructed in 2 months,according to the results of OC and TRAP levels and cross-sectional observation of the distal femur in rats.After administration in different ways and concentrations,OC level increased（P〈0.01） but TRAP level decreased（P〈0.05） in two osteoporotic model groups comparing with those in model groups.Histological observation showed that new trabecular bone formed,neatly arranged,thickened,and the number increased in 2 groups after drug administration.Conclusion GBE promotes bone formation in osteoporotic rats in both intragastric administration and intraperitoneal injection.It promotes OC secretion by osteoblasts and inhibits TRAP secretion by osteoclasts.It promotes the formation of trabecular bone and increases bone mineral density.The effect is directly related to the route of administration and dosage.