转基因大豆DNA提取方法研究

Study on DNA Extraction Method of Genetically Modified Soybean

为保障广大消费者在转基因大豆食品安全上的知情权和选择权,以及对转基因大豆的检测和标识性管理,本研究采用传统CTAB方法和改良的SDS法对大豆基因组DNA提取方法进行比较,并对相关内源性基因和外源基因进行PCR扩增。结果显示改良的SDS法在DNA提取量和完整性方面均优于传统CTAB;可在PCR检测转基因大豆中以大豆内源基因Lectin为内对照对其进行扩增,从而可有效检测空白对照中是否有DNA的污染来排除由模板中杂质造成的假阴性结果。

For the protection of consumers on food safety of genetically modified soybean＇s right to know and the right to choose, and the genetically modi fled soybean detection and identification of management, the traditional CTAB method and modified SDS method of soybean genome DNA extraction methods were compared in the study, and the related endogenous genes and foreign genes were amplified by PCR. Results showed that modified SDS method was superior to the traditional CTAB in DNA extraction and complete degree; the extraction DNA could be used in the PCR detection of genetically modified soybean with the endogenous gene lectin as internal control for the amplification. which could etTectively detect blank controls in the DNA of pollution to exclude the false negative results caused by impurities in the template.