比率荧光探针的构建及应用

The Construction and Application of Ratiometric Fluorescent Probes

比率荧光法是通过测量两个不同波长处的荧光强度比值作为信号参量,以此来测定目标物的一种分析方法。由于所测得的荧光比值信号不受光源的强度和仪器灵敏度的影响,因此比率荧光探针具有较高的灵敏度、选择性和线性范围。文章中总结了基于有机荧光探针、纳米荧光探针、复合探针等几种荧光比率探针的构建模式,并对其在金属离子和有机物等典型分析物中的应用进行了综述。

Ratiometric measurments involve the observation of exchanges in the ratio of the intensity of the adsorption or the emission at two wavelengths. They permit signal rationing and thus increase the dynamic range and provide built-in correction for environmental effects. In the present work, the construction models based on fluorescent dyes, fluorescent nanoparticles or composites for ratiometric fluorescent probes were reviewed. In the meanwhile, the application of ratiometric fluorescent probes for detection of metal ions or organic pollutant was summarized.