五个不同厂家猪瘟活疫苗免疫效果对比试验

Comparison of the Immune Efficocy of Classical Swine Fever Live Vaccines from Five Different Biopharmaceutical Companies

对市面上常见的5个厂家生产的猪瘟活疫苗进行免疫效果比较试验,并通过兔体热反应法比较不同疫苗间的效价。选取75头30日龄未免疫猪瘟疫苗的健康仔猪,根据不同的母源抗体水平进行分组免疫,于免前及一免后7、14、21、28、35、42 d,二免后7、14、21、35、49、63、77、91、126 d进行采血并进行抗体检测,比较不同厂家猪瘟活疫苗的免疫效果。结果表明,A、B厂家的疫苗效价最高,其次为C、E厂家疫苗,最后为D厂家疫苗;不同厂家的猪瘟活疫苗免疫不同水平母源抗体的仔猪,所产生的免疫效果为A厂家疫苗最优,表现出较高的抗体水平和较长的持续期,其后依次为C、B、E、D厂家疫苗。

This study was carried out to estimate the immune effects of five widely used live vaccines against classical swine fever virus （CSFV） produced by five biopharmaceutical companies. The vaccines titer was determined and compared by using rabbit fever test. Seventy-five 30-day-old and CSFV-negative pigs were randomly selected, and then divided into groups for immunization based on their maternal antibody levels. Blood samples were collected and tested for antibodies before immunization and at day 7, 14, 21, 28, 35, 42 after the first immunization, and day 7, 14, 21, 35, 49, 63, 77, 91, 125 after the second immunization. The immune effects from different biopharmaceutical companies were compared and evaluated consequently. The results demonstrated that the vaccines from company A and B were the highest titer, followed by the vaccines from company C and E, while the vaccine from company D showed the lowest titer. The vaccine from company A showed the best immune effect, the highest level of antibodies, and the greatest stability, followed by the vaccines from company C, B, E, D.