基于离心微流技术的阵列化检测芯片

An Array Detection Chip Based on the Centrifugal Microfluidic Technology

针对医疗行业多指标检测的需求,利用微流控技术高通量的优势,设计出一款由离心力驱动的阵列化检测芯片。首先介绍了芯片所涉及的力学理论,并由此计算出阀的突破频率,然后用COMSOL软件仿真流体运动,验证突破频率的正确性以及芯片结构的合理性,最后用软光刻技术和模塑法制得PDMS芯片进行离心实验,观察流体实际运动情况并和仿真结果作对比。经验证,力学理论计算所得突破频率与仿真结果和离心实验结果吻合,说明疏水阀设计的正确性。此微流控芯片实现了15通道的阵列化探测,此外还具有加样方便、结构简单等优点。

Aimed at meeting the multi-criteria detection in the medical industry,an array detection chip drived by the centrifugal force was designed using the high throughout advantage of the microfluidic technology.The mechanics theory involved in this chip was firstly introduced,and the valve burst frequency was calculated by this theory.Then the fluid motion was simulated by the COMSOL software,validating the correctness of the burst frequency and the rationality of the chip structure.Finally,the PDMS chip was fabricated using the soft lithograph and molding method,and the centrifugal test was carried out to observe the physical fluid motion and then was made contrast with the simulation result.By verification,the burst frequency calculated by the mechanical theory is consistent with the simulation result and centrifugal test result,indicating the correctness of the hydrophobic valve design.In addition,the array detection of the fifteen channels was realized in this microfluidic chip.The chip also has advantages of adding sample conveniently and simple structure.