低氧微环境对人肺癌细胞上皮间充质转化及迁移侵袭能力的影响

The Influence of Hypoxia Microenvironment on Metastasis Induced by Epithelial-Mesenchymal Transition of Human Lung Adenocarcinoma

目的探讨低氧微环境对肺癌细胞H460上皮间充质转化的影响。方法常氧和低氧(1%)培养人肺腺癌细胞系H460,分别用显微镜观察细胞形态的变化,采用Western blot检测上皮间充质转化标志物E-cadherin和Vimentin蛋白水平的变化,通过Transwell迁移和侵袭实验观察细胞迁移和侵袭能力的改变。结果常氧组的H460细胞呈不规则的多边形,而低氧组细胞出现纺锤形改变,部分细胞变得更加瘦长。与常氧组比较,低氧组的上皮标志蛋白E-cadherin表达下调,而间质标志蛋白Vimentin增加;低氧微环境使细胞的迁移和侵袭能力都得到了增强。结论低氧微环境促进了肺癌细胞的上皮间充质转化,并增强了其转移能力。

Objective To investigate the influence of hypoxia on pro-metastasis induced by epithelial-mesenchymal transition （EMT） of human lung adenocarcinoma. Methods The human lung cancer cell line H460 was cultured in hypoxic condition and the morphologic changes of the cells were observed under the microscope. The EMT-related markers including E-cadherin and vimentin were detected by Western blot. Transwell migration assay and transwell invasion assay were employed to detect the migratory and invasive activity of cancer cells. Results Hypoxic induced morphological changes were consistent with the mesenchymat phenotype, such as an elongated fibroblastic morphology, and conversion from a tightly packed epithelial cobblestone pattern to a loosely packed scattered phenotype. Compared with the control group, hypoxic attenuated the quantity of E-cadhenrin, but increased vimentin, which resulted in promotion of migration and invasion of H460. Conclusion Hypoxia induces EMT in H460 and enhances the invasive and migratory abilities of lung cancer cells.