宫颈癌患者外周血淋巴细胞亚群分布的差异分析

Analysis of the differences of distribution of lymphocyte subsets in peripheral blood in the patients with cervical cancer

目的检测分析宫颈癌患者外周血淋巴细胞亚群的差异分布及临床意义。方法采用流式细胞术检测宫颈癌患者和健康对照成人外周血中CD3^+、CD3^+CD4^+、CD3^+CD8^+、NK、NKT、CD8^+CD28^+、CD8^+CD28-、CD3^+HLA-DR^+、CD3-HLA-DR^+、Treg细胞的百分比,同时结合宫颈癌患者的临床诊断分期做对比分析。结果与健康对照组比较,宫颈癌患者外周血中CD3^+(P=0.0002)和CD3^+CD4^+T细胞百分比(P=0.0004)以及CD3^+CD4^+/CD3^+CD8^+比值(P=0.0256)显著降低;宫颈癌患者CD3^+CD8^+T细胞百分比与对照组比较无显著差异,但是随着患者临床分期的提高呈降低趋势(P=0.0480)。NK细胞(P<0.0001)、活化T细胞(CD3^+HLA-DR^+:P<0.0001)、活化B细胞(CD3-HLA-DR^+:P=0.0021)百分比显著提高;细胞毒性T细胞(CD8^+CD28^+)细胞百分比降低(P=0.0006),抑制性T细胞(CD8^+CD28-)百分比提高(P=0.0009);CD4^+CD25^(bright)T细胞(Treg)阳性细胞百分比与对照组比较没有显著差异,但CD4^+CD25^(bright)T细胞(Treg)在CD4^+淋巴细胞中的百分比显著高于对照组(P=0.0335)。宫颈癌患者外周血中CD8^+CD28-/CD8^+CD28^+之比与对照组比较显著提高(P<0.0001)。结论宫颈癌患者细胞免疫功能降低。精确分析宫颈癌患者的T淋巴细胞亚群变化对于临床诊断、治疗和预后评估具有重要的实际意义。

Objective To test and analyze the differences of distribution of lymphocyte subsets in peripheral blood in the patients with cervical cancer and its clinical significance. Methods Percentages of CD3^＋, CD3^＋CD4^＋, CD3^＋CD8^＋, NK, NKT, CD8^＋CD28^＋, CD8^＋CD28^-, CD3^＋HLA-DR＋, CD3^-HLA-DR^＋, Treg cells were tested in adult peripheral blood in the patients with cervical cancer and health controls via flow cytometry. Comparative analysis was conducted in combination with the clinical diagnosis staging in the patients with cervical cancer. Results Compared with the healthy control group, the percentages of CD3^＋（P=0.0002） and CD3^＋CD4^＋ T cells （P=0.0004） in the peripheral blood in the patients with cervical cancer and CD3^＋CD4^＋/CD3^＋CD8^＋ ratio（P=0.0256） were significantly reduced; the percentages of CD3^＋CD8^＋ T cells in the patients with cervical cancer were not statistically significant compared with that in the control group, but with the increase of clinical staging, the percentage was in a decreasing trend（P=0.0480）. The percentages of NK cells （P〈0.0001）, activated T cells （CD3^＋HLA-DR＋：P〈0.0001）, and activated B cells （CD3^-HLA- DR＋： P=0.0021） were significantly improved; the percentage of toxic T cells （CD8^＋CD28^＋） was reduced （P=0.0006）, and the percentage of inhibitive Y cells（CD8^＋CD28^-） was increased（P=0.0009）; the percentage of CD4^＋CD25^＋ （Treg） cells was not significantly different from that in the control group, but the percentage of CD4^＋CD25^brigh （Treg） cells in CD4^＋ lymphocytes was significantly higher than that in the control group （P=0.0335）. CD8^＋CD28^-/CD8^＋CD28^＋ ratio in the peripheral blood in the patients with cervical caner was significantly increased compared with that in the control group （P〈0.0001）. Conclusion Cell immunological function is reduced in the patients with cervical cancer. Accurate analysis of the changes of T lymphocyte subsets in the patients with cervical cancer has important and practical significance in clinical diagnosis, treatment and prognosis evaluation.