薏苡仁油纳米粒的质量评价

Quality evaluation of Coicis Semen oil nanoparticles

目的对薏苡仁油纳米粒进行质量评价。方法透射电镜观察纳米粒的形态,纳米粒度仪测定粒径及Zeta电位,TLC法鉴别特征成分,GC法测定油酸与亚油酸含有量（两者性质不稳定,故先测定其甲酯化产物油酸甲酯和亚油酸甲酯含有量,再换算成油酸与亚油酸含有量）,超高效液相色谱-质谱（UPLC-MS）法测定甘油三油酸酯含有量。结果所制备的纳米粒圆整光滑,大小均匀,分散性能较好。平均粒径为（193.3±0.8）nm,平均Zeta电位为-（34.40±5.82）m V。TLC斑点清晰,专属性强。油酸甲酯、亚油酸甲酯、甘油三油酸酯分别在0.025 2～0.806 4、0.021 3～0.680 3、0.142 0～15.62μg/m L范围内线性关系良好,平均回收率分别为97.18%（RSD=1.13%）、100.9%（RSD=0.71%）、100.8%（RSD=1.84%）。结论该方法简便准确,可用于控制薏苡仁油纳米粒的质量。

AIM To evaluate the quality of Coicis Semen oil nanoparticles. METHODS The nanoparticles had their morphologies check under transmission electron microscope,and their particle sizes and Zeta potentials were determined by nanoparticle size analyzer. TLC was applied to identifying the characteristic constituents,GC was used for determining the contents of methyl oleate and methyl linoleate,the methyl esterification products of unstable oleic acid and linoleic acid through which the contents of oleic acid and linoleic acid were obtained after calculation. And ultra performance liquid chromatography-mass spectrometry（ UPLC-MS） was adopted in the quantitative determination of glycerol trioleate. RESULTS The prepared nanoparticles were round and smooth with uniform size and good dispersion performance,whose average particle size was（ 193. 3 ± 0. 8） nm,and average Zeta potential was-（ 34. 40 ± 5. 82） mV. The clear TLC spots proved the strong specificity of the test. Methyl oleate,methyl linoleate and glycerol trioleate showed good linear relationships within the ranges of 0. 025 2-0. 806 4 mg / mL,0. 021 3- 0. 680 3 mg / mL and 0. 142 0- 15. 62 μg / mL, whose average recoveries were97. 18%（ RSD = 1. 13%）,100. 9%（ RSD = 0. 71%） and 100. 8%（ RSD = 1. 84%）,respectively. CONCLUSION This simple and accurate method can be used for the quality control of Coicis Semen oil nanoparticles.