不同强度静磁场促进成骨细胞增殖和分化的研究

Effects of Static Magnetic Field at Different Intensity on Proliferation and Differentiation of Osteoblast Cells

目的:研究不同强度磁场对成骨细胞增殖与分化的影响,为寻求合适的静磁场作用强度提供实验基础。方法:体外培养Wistar大鼠颅骨成骨细胞,分别置于0Gs,300Gs、1400Gs、2000Gs不同磁场强度的静磁场中作用。在1、3、5、7d用CCK-8法检测成骨细胞的增殖情况,流式细胞仪测定不同磁场强度下48h时细胞所处的周期,q-PCR检测磁场作用下7d成骨细胞内成骨因子OPN,Runx2表达情况。结果:结果显示各组均出现不同程度的促增殖作用,尤以300Gs组作用7d时显著。300Gs磁场作用组S期和G2/M期细胞百分比显著增加,推测一定磁场强度可促使静止态的G0/G1期细胞活跃向S期转变,细胞增殖加速。在300Gs磁场作用7d后,成骨因子OPN,Runx2表达都显著增高。结论:实验采用的300Gs强度磁场可有效促进成骨细胞的增殖与分化。

Objective：To observe the effects of different intensity static magnetic fields（SMFs）on the proliferation and differentiation of rat osteoblast cells.Methods：Rat calvarias osteoblasts were cultured and incubated in the SMFs of 0Gs,300 Gs,1400Gs and 2000 Gs.CCK-8method was used to detect osteoblast proliferation at day 1,3,5and 7.Flow cytometry was employed to detect the cell cycle of osteoblasts under different intensity magnetic fields.Expressions of osteogenic marker genes like OPN and Runx2 were measured by quantitative polymerase chain reaction.Results：The proliferation of osteoblasts exposed to 300 Gs SMF for 7days increased obviously with significant difference as compared with 0Gs group（P 〈0.05）.Moreover,after magnetic exposure with intensity of300 Gs,G0/G1 phase percentage decreased,with S phase and G2 phase percentage significantly increased as compared with the control.The expression of OPN and Runx2 in osteoblast exposure to 300 Gs and 2000 Gs were significantly higher than the control group,which suggested that magnetic field of intensity of 300 Gs had the ability to induce osteogenic differentiation of osteoblasts.Conclusion：Static magnetic field of intensity of 300 Gs could increase the proliferation and differentiation of osteoblasts.