人β-防御素-3对小鼠牙周炎进展的保护作用

Protective Effect of Human β-defensin-3 on the Progression of Periodontitis

目的:探索人β-防御素-3在小鼠牙周炎进展中的保护作用。方法:用丝线结扎法建立小鼠牙周炎模型。实验组小鼠腹腔注射人β-防御素-3(100μg/kg),另选结扎过小鼠和未结扎小鼠各1组,腹腔注射磷酸盐缓冲液为对照。8周后取上颌骨,Micro CT、HE染色、TRAP染色及免疫组化染色,评估人β-防御素-3对牙周炎进展的作用。结果:人β-防御素-3能减少牙槽骨吸收及骨内破骨细胞数量;减轻牙龈上皮棘层增厚、上皮钉突增生、炎症细胞浸润、胶原纤维变性断裂等病理变化,降低牙周组织中IL-6、MCP-1和TNF-α的表达强度。结论:人β-防御素-3能够减轻牙周组织炎症,减少牙周组织破坏,从而减缓牙周炎进展。

Objective：To investigate the effect of human β-defensin-3on the progression of periodontitis.Methods：Periodontitis models in mice were established through ligating the maxillary second molar with 5-0silk thread.Mice in the experimental group were given human β-defensin-3intraperitoneally with a dose of 100μg/kg.Mice with or without ligation were given phosphate buffer solution intraperitoneally as the control.Eight weeks later,the maxilla of each mouse was harvested and detected by Micro-CT scanning,HE staining,TRAP staining,and immunostaining.Results：Human β-defensin-3reduced the alveolar bone loss and the number of osteoclast in alveolar bone.In addition,human β-defensin-3alleviated the pathologic changes including thickened spinous layer,hyperplastic epithelial spikes,inflammatory cell infiltration,denatured and broken collagen fibers caused by ligation.Furthermore,lower IL-6,MCP-1and TNF-αexpressions were observed in mice treated with human β-defensin-3.Conclusion：Human β-defensin-3alleviates the inflammation and destruction in periodontal tissue,thus slowing down the progress of periodontitis.