‘黄花倒水莲’离体快繁技术研究

The technology research in vitro rapid propagation of Polygala fallax Hemsl

【目的】研究并建立‘黄花倒水莲’离体快繁技术体系.【方法】以‘黄花倒水莲’嫩茎为外植体,经不同方法消毒后,接种于MS+6-BA 2.0mg/L+NAA 0.05mg/L的培养基中诱导不定芽发生,继而将不定芽接种在附加不同种类及浓度外源激素的培养基进行培养,根据增殖和生根情况,筛选适宜的‘黄花倒水莲’增殖及生根培养基;然后将生根试管苗移栽于不同的基质中,依据成活率,确定其最佳炼苗基质.【结果】用75%酒精消毒10s,0.1%升汞消毒15min获得了较好的消毒效果,污染率低至3.15%;适宜‘黄花倒水莲’增殖的培养基为MS+6-BA1.5mg/L+NAA 0.05mg/L,增殖系数为5.50;在1/2MS+IBA 0.1mg/L+NAA 0.3mg/L+0.2g/L活性炭的生根培养基中,其生根率可达96%;‘黄花倒水莲’试管苗炼苗的最佳基质为红壤∶腐殖土∶珍珠岩=1∶1∶1,成活率为94.5%.【结论】成功建立了‘黄花倒水莲’离体快繁技术体系.

【Objective】Technological systemin vitro rapid propagation of Polygala fallax Hemsl was studied and established.【Method】After disinfecting with different methods,the tender stems of P.fallaxused as explant were inoculated in culture medium of MS＋6-BA 2.0mg/L ＋NAA 0.05mg/L to induce the adventitious buds.Then adventitious buds were inoculated in different culture medium with different kind and concentration exogenous hormonesto to culture.The suitalble proliferation and rooting culture medium of P.fallaxwere selected by the condition of proliferation and rooting.The rooting seedllings of P.fallaxwere planted in different matrix to selected the suitable exercising matrix by statistic of survival rate.【Result】Better sterilization effect was obtained by the method of disinfected with 75%alcohol for 10 s and 0.1% mercuric chloride for 15 min.The contamination rate was as low as 3.15%.MS＋6-BA1.5mg/L＋NAA 0.05mg/L was suitable for P.fallaxproliferation culture,the proliferation confficient was 5.50;1/2 MS＋IBA 0.1mg/L＋NAA 0.3mg/L＋0.2g/L activated carbon was suitable for P.fal-laxrooting culture.The rooting rate was 96%;The best exercising matrix of P.fallaxvitro seedlling is laterite∶humus∶perlite=1∶1∶1,the survival rate is 94.5%.【Conclusion】The vitro rapid propagation of P.fallax was established triumphantly.