皮肤胆固醇无创光谱检测模拟和在体实验研究

Simulation and in vivo Experimental Study on Noninvasive Spectral Detection of Skin Cholesterol

皮肤胆固醇是评估动脉粥样硬化性疾病风险的新型生物标志物。为实现皮肤胆固醇的无创、快速检测，设计了一套基于漫反射光谱技术的检测系统。采用毛地黄皂苷一辣根过氧化物酶共聚物溶液模拟梯度浓度皮肤胆固醇。通过检测该共聚物溶液和受试者在体皮肤胆固醇，验证了系统的可行性。结果表明，在440～550 nm特征波段内，由相对漫反射率引人的参数S值与共聚物溶液浓度呈线性关系（相关系数r=0．992，P〈0．01）；控制年龄、性别等因素后，受试者在体皮肤胆固醇无创检测结果与血液中总胆固醇和低密度脂蛋白胆固醇呈显著正相关，相关系数r分别为0．837（P〈0．01）和0．778（P〈0．01）。漫反射光谱技术为皮肤胆固醇的检测提供了一种无创、便捷的方法。在体皮肤胆固醇的无创检测有助于动脉粥样硬化性疾病的早期发现。

Skin cholesterol is a novel biomarker to assess the risk of atherosclerotic diseases. A system based on diffuse reflectance spectroscopy technology is designed for noninvasive and rapid detection of skin cholesterol. The feasibility of the system is validated through detecting digitonin-horseradish peroxidase copolymer solution which simulates the skin cholesterol of gradient concentration and skin cholesterol in vivo of subjects. A parameter S based on the relative diffuse reflectance increases linearly with the increasing concentration of the copolymer solution in the wavelength band from 440 nm to 550 nm （correlation coefficient r=0.992, P〈0.01）. After adjusting age, gender and other factors, it shows significant positive correlation between the skin cholesterol test results of subjects and the total cholesterol, low density lipoprotein cholesterol, and the correlation coefficients are 0. 837 （P〈0.01）and 0. 778 （P〈0.01）, respectively. The diffuse reflectance spectroscopy is a noninvasive and convenient method for the detection of skin cholesterol, and the noninvasive detection of skin cholesterol in vivo contributes to the early detection of atherosclerotic diseases.