内源IAA、GA_3调控对百合试管苗生长的影响研究

Influence of Endogenous IAA and GA_3 on in vitro Lily Seedling Growth

以东方百合‘Sorbonne’试管苗为对象,对不同浓度IAA及其抑制剂(TIBA)添加后试管苗茎叶和根的生长情况进行了研究,测定了试管苗中内源激素(IAA,GA_3,Zeatin)的变化。数据显示10 mg·L^(^(-1))IAA促进试管苗茎叶、根生长的效果最佳而40 mg·L^(^(-1))IAA抑制试管苗的生长,TIBA抑制试管苗茎叶、根生长的效果显著。内源激素测定结果表明外源IAA及TIBA处理影响了内源激素的变化,IAA对生长的影响可能是通过调节内源激素的变化而实现的。结合生长情况及内源激素变化的分析结果表明:过高浓度的IAA、过低浓度的GA_3都不利于试管苗生长,内源IAA和GA_3在适宜浓度下才能发挥促进生长的作用;内源Zeatin在试管苗的生长调控中可能不发挥作用。本实验为百合种植中外源激素的合理使用提供了依据,有助于提升百合种植者的经济效益。

The in vitro seedlings of Lilium oriental ＇ Sorbonne＇ were used to study the response of plants to exogenous IAA and its inhibitor （2,3,5-Triiodobenzoic acid, TIBA）, including the growth of stems, leaves and roots, and the dynamic change of endogenous hormones was analyzed by HPLC. The data showed that 10 mg · L-1 IAA have the best significant role on growth of stems, leaves and roots simultaneously while 40 mg· L-1 IAA and TIBA treatments inhibited growth significantly. By the hormone analysis, the endogenous hormones was influenced by exogenous IAA and TIBA application, the effect of IAA on growth was achieved probably by endogenous hormones regulation. Analysis based on the growth and the change of endogenous hormones revealed that higher level of endogenous IAA and lower level of GA3 were disadvantageous for growth, and endogenous IAA and GA3 played optimal role in their optimum concentrations. Endogenous Zeatin perhaps did not take part in the regulation of seedling growth. These results would be helpful in the rational use of exogenous hornaones in lily planting and useful to improve economic benefit of growers.