摘要
家禽卵巢中颗粒细胞在卵泡发育和闭锁中起着重要作用,包括信号传递、营养供给以及离子平衡等。卵泡颗粒细胞的增殖与分化自身也受到生殖激素和细胞因子的综合调节,其作用机制是当前研究热点。家禽卵泡颗粒细胞的体外培养可作为研究繁殖生理调节的理想细胞模型。本文对家禽卵泡颗粒细胞的体外培养研究现状及颗粒细胞在卵泡生长发育和闭锁中的作用进行简要概述,并总结了原代颗粒细胞体外培养模型的建立方法。
Granulosa cells play an important role in the development and atresia of follicles in poultry,including mediating signal transduction,supplying nutrients for oocyte,and ion balance regulating,etc.Varieties of reproductive hormones and cytokines determine the fate of follicle by affecting the proliferation and differentiation of granulosa cells,and the related mechanism is of great interest for research.Culture in vitro of granulosa cells provides an idea way in studying fowl reproductive physiology regulation.In this review,we overviewed the research progress of granulosa cells culture in vitro of fowl follicle and the role of granulosa cells in follicular development and atresia,and summarized the method of primary culture in vitro for follicular granulosa cells.
作者
罗茜
陈伟
郑春田
林映才
王胜林
阮栋
王爽
夏伟光
邱月琴
熊云霞
LUO Xi CHEN Wei ZHENG Chuntian LIN Yingcai WANG Shenglin RUAN Dong WANG Shuang XIA Weiguang QIU Yueqin XIONG Yunxia(Institute of Animal Science, Guangdong Academy of Agricultural Sciences, State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2016年第9期2681-2686,共6页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金(31301995)
现代农业产业技术体系建设专项资金资助(CARS-43-13)
关键词
家禽
卵泡颗粒细胞
体外培养
fowl
follicle granulosa cells
in vitro culture
