植物细胞程序性死亡检测技术研究进展

Research Progress on Genetic Engineering for Long-chain Polyunsaturated Fatty Acids EPA and DHA

细胞程序性死亡（PCD）是一种细胞生物学反应,广泛存在于植物的生长发育与抗逆过程,是当今生命科学的研究热点之一。从形态学、生物化学和分子生物学等方面综合分析了植物细胞PCD检测方法的研究进展,并对现有植物PCD检测技术的不足和解决途径进行了讨论与展望,以期为后续PCD研究和应用提供借鉴。

Long-chain polyunsaturated fatty acids（LCPUFAs）play an important role in maintaining human health. The demand of edible EPA and DHA has significantly increased in recent years. However,the fish oil resource is obviously reduced due to the environmental pollution and the decline of the fishery resources,thus can’t meet the demands of people. The researchers have successively separated multiple genes that involved in LCPUFA synthesis and clarified the multiple LCPUFA synthetic pathways. LCPUFAs,especially DHA and EPA,have been successfully produced in monocot and dicot plants through genetic engineering. We summarized the latest research progress of LCPUFAs bio-synthesis and genetic engineering in higher plants,and analyzed the issues of LCPUFA synthesis,then proposed the possible solutions,and had an prospect of LCPUFA by genetic engineering.