鸭脂肪间充质干细胞分离培养与生物学特性

Isolation and Biological Characterization of Duck Adipose-derived Mesenchymal Stem Cells

建立禽类脂肪来源的间充质干细胞(adipose-derived stem cells,ADSCs)体外培养体系,探索其生物学特性和多分化潜能。通过酶消化法分离18日龄鸭胚ADSCs,绘制生长曲线,通过RT-PCR、免疫荧光和流式细胞术阳性率检测对ADSCs进行鉴定,诱导ADSCs向成骨细胞和脂肪细胞分化。结果表明,鸭胚胎ADSCs体外增殖能力良好;阳性表达CD29,CD44,CD105,表达率分别为95.39%、94.53%和98.19%;经体外诱导后可分化为成骨细胞和脂肪细胞。研究认为在适宜的培养条件下,体外培养的ADSCs能够稳定增殖并具有多分化潜能,可作为组织工程的种子细胞进行保存。

This study aims to establish in vitro culture system for the poultry adipose-derived stem cells（ADSCs）,to study the biological characteristics and the multi-differentiation potential. Enzyme digestion method was applied to separate Beijing duck 18-day-old embryos ADSCs and to draw the growth curve. ADSCs were identified by RT-PCR,immunofluorescence and flow cytometric analysis. Our results suggested that the ADSCs had solid proliferative activity and were positive for CD29,CD44 and CD105,the rate of CD29＋, CD44＋,and CD105＋ ADSCs was 95.39%,94.53%,98.19%,respectively. ADSCs differentiated into adipocytes and osteoblasts by induced differentiation in vitro. In summary,under appropriate experimental circumstances,Beijing duck embryo ADSCs have a strong self-renewing ability and multi-differentiation potential in vitro,and it can be an ideal type of seed cell for cellular transplantation therapy.