贮藏温度和烟叶含水率互作对白肋烟贮藏期间TSNAs形成的影响

Interaction of storage temperature and moisture content in tobacco leaf on TSNAs formation in burley tobacco during storage

为揭示贮藏条件对白肋烟贮藏过程中烟草特有亚硝胺（TSNAs）形成的影响规律,将不同含水率（7.71%、15.30%和22.10%）的烟叶分别置于10℃、20℃、30℃、40℃和50℃条件下贮藏15 d后测定TSNAs含量（质量分数）。结果表明：贮藏温度和烟叶含水率对TSNAs的形成具有显著影响,且两者间存在显著的交互效应,其中温度是烟叶TSNAs总变异的主要影响因子,贡献率达56.1%;其次是烟叶含水率,贡献率为25.6%;两者互作的贡献率为18.2%。温度和含水率的双因素模型较好地模拟了TSNAs对两因素的响应,贮藏温度和烟叶含水率共同解释了贮藏过程中烟叶TSNAs变异的93.2%～96.5%。为了有效地抑制贮藏过程中烟叶TSNAs的形成,建议白肋烟贮藏温度低于25.5℃且烟叶含水率≤18%。

To evaluate the effects of storage condition on TSNAs formation in burley tobacco during storage, tobacco leaves with different moisture contents （7.71%, 15.30% and 22.10% ） were stored at different temperatures （10 ℃, 20 ℃, 30 ℃, 40 ℃and 50 ℃） separately for 15 days, and the content of TSNAs in the leaves was determined. The results showed that storage temperature and the moisture content in tobacco leaves significantly influenced TSNAs formation and significant interactive effects were found between storage temperature and the moisture content. Storage temperature, as the main factor of total variation of TSNAs, had contribution rate of 56.1%; the contribution rate of moisture content was 25.6%, and that of their interaction effect was 18.2%. The bifactorial model of temperature and moisture content simulated the response of TSNAs to storage temperature and moisture content pretty well. Storage temperature and the moisture content in leaves explained 93.2%-96.5% variations in TSNAs formation during storage. To effectively inhibit TSNAs formation during tobacco storage, it is suggested that the appropriate storage temperature for burley tobacco should be below 25.5 ℃ and the moisture content in leaves should be not above 18%.