潍坊市城区2015年淡色库蚊对常用杀虫剂的抗药性现状调查

Investigation on Culex pipiens pallens resistance to commonly used insecticides in Weifang city in 2015

目的了解山东省潍坊市城区淡色库蚊对3种常用杀虫剂的抗药性水平,为科学使用杀虫剂提供依据。方法 2015年8月7-10日,在潍坊市4个区采集淡色库蚊卵块,带回实验室饲养;采用浸渍法测定淡色库蚊3龄末4龄初幼虫对杀虫剂的半数致死浓度（LC_（50））。结果潍坊市城区淡色库蚊幼虫对双硫磷的LC（50）为0.003 466~0.005 472 mg/L,为敏感品系的7.12~11.24倍;对溴氰菊酯的LC_（50）为0.000 908~0.001 866 mg/L,为敏感品系的1.86~3.83倍;对高效氯氰菊酯的LC_（50）为0.001 798~0.006 097 mg/L,仅为敏感品系的0.47~1.60倍,处于敏感水平。结论潍坊市城区淡色库蚊对双硫磷为中抗,对溴氰菊酯为低抗或敏感,对高效氯氰菊酯敏感。应避免单一长期使用双硫磷,不同种类杀虫剂交替轮用或复配使用,预防和减缓抗药性的产生。

Objective To monitor the resistance status of Culex pipiens pallens to three kinds of commonly used insecticides in Weifang city for management of insecticide resistance development. Methods Egg rafts of Cx. pipiens pallens were collected from four districts in Weifang, and reared to larvae in insectary. The late-third-to-early-fourth-instar larvae were tested against temephos, dehamethrin and beta-cypermethrin with dipping method to determine the median lethal concentration （LCs0）. Results The resistance ratios （RRs） to temephos ranged from 7.12 to 11.24 （LC50=0.003 466-0.005 472 mg/L）; to deltamethrin ranged from 1.86 to 3.83 （LC50=0.000 908-0.001 866 mg/L）; to betacypermethrin were 0.47-1.60 （LC50=0.001 798-0.006 097 mg/L）. Conclusion Culex pipiens paUens has developed resistance to temephos, tolerance to deltamethrin, and remained susceptible to beta-cypermethrin. To prevent and control the development of insecticide resistance, insecticides including temephos should be used in rotation or mixture with other pesticides with different modes of action, while avoiding using single active ingredient for long time.