摘要
为探究马链球菌马亚种(Streptococcus equi subsp.equi,S.equi)对小鼠骨髓源树突状细胞(bone marrow derived dendritic cells,BMDCs)TLR1、TLR2、TLR6、MyD88、IL-1、IL-6、IL-10、IL-12、TNF-αmRNA表达的影响,用GM-CSF、IL-4细胞因子刺激小鼠骨髓细胞使其诱导分化成未成熟的BMDCs,感染S.equi后16、20、26h,采用SYBR GreenⅠ实时荧光定量PCR检测感染组及未感染组细胞TLRs、MyD88和细胞因子mRNA的表达情况。结果显示,S.equi感染BMDCs后16、20、26h,TLR1、TLR2、TLR6、MyD88mRNA的表达量均显著或极显著的高于未感染组(P<0.05或P<0.01)。S.equi感染DCs后16、26h,IL-1的分泌量较未感染组没有变化(P>0.05),IL-10、TNF-α的分泌量显著或极显著增加(P<0.05或P<0.01)。IL-6在S.equi感染DCs后16h的分泌量显著增加(P<0.05),但感染后26h无显著变化(P>0.05)。IL-12在S.equi感染DCs后16h的表达没有变化(P>0.05),但感染后26h分泌量有所增加(P<0.05)。说明S.equi具有调节DCs TLRs、MyD88和细胞因子表达的能力,从而介导机体的免疫应答反应。
To study the effects of Streptococcus equi subsp equi （S. equi） on the mRNA expressions of TLR1, TLR2, TLR6, MyD88, IL-1, IL-6, IL-10, IL-12 and TNF-α in mouse bone marrow derived dendritic cells （BMDCs） cultured in vitro ,Naive DCs were cultured with GM-CSF and IL-4,SYBR Green Ⅰ fluorescence quantitative PCR was used to detect mRNA transcriptional levels of TLRs,MyD88 and cytokines after infection with S. equi about 16,20,26 h. The results showed that at 16,20,26 h post-infections, the expression levels of TLR1, TLR2, TLR6 and MyD88 in S. equi infected BMDCs were significantly or extremely significantly higher than those of the negative control group （P〈0.05 or P〈0.01 ）. At 16,26 h post-infections, the concentration of IL-1 had no obviously change （P〈0.05）, IL-10, TNF-α were significantly or extremely significantly increased than those of the negative control group （P〈0.05 or P〈0.01）. IL-6 was significantly increased at 16 h post-infection （P〈0. 05）, however,it had no change at 26 h postinfection （P〉0. 05）. At 16 h post-infection, the concentration of IL-12 had no obviously change （P〉 0.05） ,while at 26 h post-infection,it was significantly increased （P〈0.05）. These results indicated that S. equi was able to regulate the mRNA expressions of TLRs, MyD88 and cytokines, which mediated immune responses.
出处
《动物医学进展》
北大核心
2016年第10期16-20,共5页
Progress In Veterinary Medicine
基金
国家科技支撑计划项目(2012BAD46B00)
