桑黄黄酮液体发酵培养基的优化

Optimization of Liquid Medium for Fermenting Flavones from Phellinus linteus

研究了不同碳源、氮源对桑黄（Phellinus linteus）液体培养中胞内黄酮产量的影响。通过单因素试验筛选出最佳碳源为葡萄糖，最佳氮源为黄豆粉；利用L9（3^4）正交试验，初步筛选出了桑黄胞内黄酮液体发酵优化培养基为玉米粉2．0％、葡萄糖1．5％、黄豆粉1．0％、蛋白胨1％、酵母膏0．5％、KH2PO40．2％、MgSO40．05％、VB110mg／100mL。

The effect of different carbon sources, nitrogen sources on the production of intracellular flavone in liquid culture of Phellinus linteus has been studied. The optimum carbon and nitrogen source has been determined by performing single factor experiment. The result shows that the optimum carbon source is glucose, and the optimum nitrogen source is soybean meal. Using L9 （3^4） orthogonal test, the optimum medium of liquid fermentation for intracellular flavonoids from P. igniarius optimization is corn flour 2.0%, glucose 1.5%, soybean meal 1%, peptone 1%, yeast extract 0.5%, KH2PO4 0.2%, MgSO4 0.05%, VBI 10 mg/100mL.