β-葡萄糖醛酸酶在胰腺癌患者血清及组织中活性变化的临床意义

Clinical Significance of the Changes of the Activity of β-G in Serum and Tissues of Pancreatic Cancer

目的联合检测胰腺癌患者血清及组织中β-葡萄糖醛酸酶(β-G)水平,分析其活性变化的临床意义。方法选择行手术治疗的40例胰腺癌患者作为实验组,同期健康体检者作为对照组,采用酶联免疫吸附法检测外周血清中β-G的水平,免疫组织化学法检测入组患者的胰腺癌组织及癌旁组织中β-G的阳性细胞百分率及阳性表达程度。结果实验组手术前血清β-G水平为(16.59±1.69)ng/ml,出院前为(9.23±1.21)ng/ml,较治疗前明显降低,但高于对照组,差异有统计学意义(P<0.05);胰腺癌组织中β-G阳性细胞百分率为(62.5±4.1)%,明显高于癌旁组织,差异有统计学意义(P<0.05);12例淋巴结转移者胰腺癌组织β-G阳性细胞百分率与无淋巴结转移者比较无显著差异(P>0.05);中低分化腺癌β-G阳性表达率高达85%,明显高于高分化腺癌,差异有统计学意义(P<0.05)。结论胰腺癌患者血清及组织中β-G水平升高,且肿瘤分化程度越低,其表达强度越强,与是否出现淋巴结转移无关。

Objective To detect the serum and tissues level of β-G in patients with pancreatic cancer, and to analyze the clinical significanee of the ehanges of the activity of β-G. Methods 40 patients with pancreatic cancer were selected as the ex- perimental group. The levels of β-G in peripheral blood were detected by enzyme-linked immunosorbent assay. The expression of β-G positive ceils in pancreatic cancer tissues and adjacent tissues were detected by immunohistoehemistry. Results Serum β-G（ 16.59 ± 1.69 ） ng/ml of the experimental group before surgery was significantly lower than before treatment, but was higher than the control group （ P 〈 0.05 ） ; The percentage of β-G positive cells in pancreatic cancer tissues （62.5± 4.1 ） % was significantly higher than that of the adjacent tissues （ P 〈 0.05 ） ; There had no significant difference in percentage of β-G positive cells be- tween 12 cases of lymph node metastasis pancreatic cancer tissues and non lymph node metastasis（ P 〉 0.05）. The positive ex- pression rate of β-G in the middle and low differentiation adenocarcinoma was 85% ,which was significantly higher than that in the high differentiated adenocarcinoma （P 〈 0.05）. Conclusion Serum and tissues levels of β-G in patients with pancreatic cancer increased, and the lower the degree of differentiation, the higher the expression intensity, and it is not related with the oc- currence of lymph node metastasis.