亚麻木酚素对复合因素诱导大鼠骨质疏松症的治疗作用研究

Study on Therapeutic Effect of Flax Lignan on Osteoporosis Induced by Combined Factors in Rats

目的:观察亚麻木酚素(FLs)对实验性骨质疏松大鼠的治疗作用。方法:60只SD大鼠随机分为正常对照组,模型对照组,阳性对照组和FLs低、中、高剂量组,除正常对照组外其余各组灌胃醋酸泼尼松,第8天灌胃维甲酸。造模第14天,FLs低、中、高剂量组大鼠分别灌胃不同剂量的FLs混悬液,阳性对照组灌胃雌二醇,正常对照组和模型对照组灌胃等容量的蒸馏水,连续14天。结果:造模后,与正常组比较模型组大鼠血清钙和磷的含量均显著降低(P<0.05,P<0.01),骨钙含量降低(P<0.05),Pmax、Mmax明显降低(P<0.05,P<0.01)。与模型组比较,FLs高剂量组股骨重量增加(P<0.05),FLs高、中剂量组大鼠血清钙和磷的含量均明显升高(P<0.05,P<0.01),FLs各剂量组骨钙含量明显升高(P<0.05),FLs各剂量都能不同程度提高Pmax,Lmax,Mmax和骨应变(P<0.05,P<0.01)。FLs各剂量组骨小梁数量增加,骨小梁厚度增厚,平均骨小梁间距减少,骨小梁体积百分比增大,平均骨皮质厚度增加(P<0.05,P<0.01)。结论:亚麻木酚素对泼尼松和维甲酸所致的大鼠骨质疏松有一定的治疗作用。

Objective： To observe the therapeutic effect of FLs on . Method： 60 SD rats were divided into the control group, the model control group, the positive control group, low dose FLs group, middle dose FLs group and high dose FLs group in random. Each group were given prednisone acetate by gavage and were given retinoic acid by garage on the 8th day except the normal control group. On the 14th day, the rats in low dose FLs group, middle dose FLs group and high dose FI,s group were given different dose FLs suspension by garage. The positive control group were given E2 by gavage. The normal control group and the model control group were given an equivalent amount of normal saline. It last 14 days continuosly. Result： After the model was built, compared with that of the normal group, the content of calcium and phosphorus in serum of the model group decreased significantly （P〈0.05, P〈0.01 ） , the content of calcium in bone of the model group decreased （P〈0.05）, Pmax and Mmax decreased significantly （ P〈0.05, P〈0.01 ） . Compared with that of the model group, the femur weight of high dose FLs group increased （ P〈0.05 ） , the content of calcium and phosphorus in serum of the high dose FLs group and middle dose FLs group decreased significantly （P〈0.05, P〈0.01 ） , the content of calcium in bone of each dose FLs group increased signitficantly （P〈0.05） , Pmax, Lmax, Mmax and stress and strain of bones increased in different degree （ P〈0.05, P〈0.01） .The quantity of bone trabecula increased, the thickness of of bone trabecula increased, the mean trabecular bone spacing（mtbs） decreased, the volume fraction of bone trabecula increased and average cortical bone thickness increased （ P〈0.05, P〈0.01 ） . Conclusion： FLs has certain therapeutic effect on osteoporosis rats due to prednisone acetate and retinoic acid.