人参醇提后药渣中人参酸性多糖的分离纯化工艺研究

Study on Separation and Purification Technology of Ginseng Acidic Polysaccharides in the Residue after Ethanol Extraction from Panax ginseng

目的:以人参醇提后的药渣制备的喷雾粉为原料,优化人参酸性多糖的分离、纯化等工艺。方法:采用以D-半乳糖醛酸为对照品计的人参酸性多糖量等为指标,优化树脂分离纯化、脱盐、干燥等制备工艺中的方法及条件,并进行小试和中试工艺验证。结果:采用D900型阴离子交换树脂分离纯化人参酸性多糖,上样量为7.2 mg/g(多糖粗品/干树脂),径高比为1∶7;先用水再用0.3 mol/L的Na Cl溶液以2倍柱体积(BV)/h的流速洗脱5 BV;超滤法脱盐,截留分子质量为1 k D;选择喷雾干燥方法制备人参酸性多糖粉末。小试验证试验中产品纯度为94.7%,中试验证试验中各指标的RSD≤2.42%(n=3)。结论 :优化所得人参酸性多糖的分离、纯化工艺稳定可靠、简便易行,适合于工业化生产,且产品纯度较高。

OBJECTIVE： To optimize the separation and purification technology of ginseng acidic polysaccharides, with the spray powder made of the residue after ethanol extraction from Panax ginseng as the raw material. METHODS： Taking the content of ginseng acidic polysaccharides, which was calculated with D-galacturonic acid as the reference substance, as the index, the method and condition in the preparation technology including resin separation and purification, desalination and drying were opti- mized, and the lab-scale test and pilot-scale test were conducted for verification. RESULTS： The Dg00 anion resin was used to puri- fy and separate ginseng acidic polysaccharides, where elution was made successively with water and 0.3 mol/L NaC1 solution at a flow rate of 2 BY/h, with a sample amount of 7.2 mg/g （crude polysaccharide/dry resin）, a diameter to height ratio of 1 ： 7 and an elution volume of 5 BV. The ultrafiltration method was used for desalination, where molecular weight cut-off was 1 kD. Spray drying was chosen for the preparation of ginseng acidic polysaccharides powder, the purity of which was 94.7% in the lab-scale test. In the pilot-scale test, the RSDs of all indexes were lower than or equal to 2.42% （n=3）. CONCLUSIONS： The optimized separation and purification technology of ginseng acidic polysaccharides is stable, reliable, simple, easy, and it is suitable for industrialized production of high-purity products.