不同加工方法对杜仲总黄酮含量的影响

Effects of Different Processing Methods on the Content of Total Flavonoids in Eucommia ulmoides

目的:比较不同加工方法对杜仲总黄酮含量的影响,为杜仲的合理化产地加工提供科学依据。方法:将新鲜杜仲制成板片和宽丝2种性状,各性状药材按"发汗"和未"发汗"进行阴干、晒干、烘干(60、80、100℃)和微波干燥(5、10、15 min)后晒干等加工方法处理制备样品。以芦丁为对照品,采用紫外分光光度法于510 nm波长测定各样品总黄酮含量。结果:不同加工方法对杜仲总黄酮含量有影响。同一干燥方法下,未"发汗"样品总黄酮含量高于"发汗"样品(P<0.05或P<0.01);同一性状、不同干燥方法下,总黄酮含量微波干燥>烘干>晒干>阴干,以微波干燥10 min后晒干样品中的总黄酮含量最高;宽丝状样品中的总黄酮含量高于板片状样品。结论:若以杜仲总黄酮的药理作用为主进行临床应用时,建议杜仲采用趁鲜切宽丝、不"发汗"、微波辅助干燥后晒干的加工方法。

OBJECTIVE： To compare the effects of different processing methods on the content of total flavonoids in Eucommia ulmoides so as to provide a scientific basis for reasonable processing of E. ulmoides in the production place. METHODS： Fresh E. ulmoides was made into flakes and shredded slices. The E. ulmoides with the two properties was classified on the basis of ＂sweating＂ and ＂without sweating＂ and dried in the shade, in the sun or by baking （60 ℃, 80 ℃ and 100 ℃ ） or dried in the sun after microwave drying （5 min, 10 rain and 15 min） for the preparation of samples. With rutin as the reference substance, ultraviolet spectrophotometry was adopted to determine the contents of total fiavonoids in all the samples at a wavelength of 510 nm. RESULTS： Different processing methods had effects on the content of total flavonoids in E. ulmoides. For the samples processed by the same drying method, the content of total fiavonoids in those without ＂sweating＂ was higher than that in ＂sweating＂ ones （P〈 0.05 or P〈0.01） ; for the samples with the same property and processed by different drying methods, the content of total fiavonoids arranged from high to low were microwave drying〉baking〉dried in the sun〉dried in the shade, where the content of total flavonoids in those dried in the sun after 10 min microwave drying was the highest; the content of total flavonoids in the shredded slices was higher than that in the flakes. CONCLUSIONS ： For clinical application of the pharmacological action of total flavonoids in E. ulmoides, it is suggested that fresh E. ulmoides cut into shredded slices and without ＂sweating＂ should be selected and dried in the sun after microwave drying.