多指标正交试验优化复方江南卷柏散提取工艺

Optimization of Extraction Technology for Compound Selaginella moellendorffii Powder with Orthogonal Test by Multiple Indicators

目的:优化复方江南卷柏散提取工艺。方法:采用多指标正交试验。以芹菜素-6-C-β-D-吡喃葡萄糖-8-C-β-D-吡喃木糖苷(YⅡ)含量、落新妇苷含量和浸膏得率的综合评分为指标,筛选最优加水倍数、提取时间和提取次数;同时进行验证试验。结果:筛选的最优提取工艺为加12倍水量提取3次,每次1 h;验证试验中YⅡ含量为0.350 6 mg/g(RSD=1.207 4%,n=3)、落新妇苷的含量为1.406 0 mg/g(RSD=1.459 8%,n=3)、浸膏得率为23.066 6%(RSD=1.660 3%,n=3)。结论:该工艺科学合理,可用于复方江南卷柏散的提取。

OBJECTIVE： To optimize the extraction technology of Compound Selaginella moellendorffii powder. METHODS： The amount of water, extracting time and times were screened by orthogonal test using the content of 6-C-β-D-glucopyrano- sul-8-C-β-D-xylopyranosyl apigonin （Y Ⅱ ） and astilbin, the yield of extract as index. The validation test was conducted. RESULTS： The optimal extraction technology was as follows as 12-fold water, decocting 3 times, 1 h each time; in validation test, the content of Y Ⅱ and astilbin were 0.350 6 mg/g （RSD=1.207 4%, n=3） and 1.406 0 mg/g （RSD=1.459 8%, n=3）; the yield of extract was 23.066 6% （RSD=1.660 3%, n=3）. CONCLUSIONS： The technology is reasonable and scientific, and can be used for the extraction of Compound S. moellendorffii powder.