副结核分枝杆菌感染的免疫应答与检测方法研究进展

Immune response of body after Mycobacterium avium subsp. paratuberculosis infection and advances in detection methods-A review

副结核分枝杆菌常引起感染牛的产奶量下降、持续性消瘦、顽固性下痢甚至死亡,给畜牧业带来了巨大的经济损失。牛通常在幼年期经口感染该菌并具有一个较长的亚临床期,后期才表现出临床症状,感染前期以细胞免疫为主并伴随着间歇排菌,经过一个2到5年的亚临床期后体液免疫应答增强,同时排菌量明显增加。目前对牛副结核病常用的检测方法有病原学检测方法,基于细胞免疫反应和基于体液免疫反应的检测方法。由于不同方法的反应原理不同,加之副结核分枝杆菌感染动物的特定免疫应答规律,在某一时间内各方法之间敏感性差异较大。本文简要阐述了牛副结核分枝杆菌的传播途径以及牛感染后的免疫应答特点,对牛副结核病的常见诊断方法进行了综述。

The progressive form of clinical Mycobacterium avium subsp, paratuberculosis （MAP） is characterized by production losses, weight loss, chronic intractable diarrhea, and severe emaciation leading to death in cattle. Substantial economic losses to the animal husbandry are a result of infection. Cattles are usually infected in their youth through the oral route and will experience a long subclinical stage. At the early stage of infection, cellular immunity is the main immune response with bacterium excretion increased significantly after a subclinical period of 2 to 5 years. The majority of methods currently used to detect MAP are based on etiological detection, cellular and humoral immune response. Owing to the different mechanism of diagnostic methods varies a lot at a particular infection period. In this review, we illustrate the transmission route and the characteristic of immune responses of MAP, and also summarize the diagnostic methods of MAP.