高氧液对急性一氧化碳中毒大鼠心肌损伤的影响

Effect of hyperoxygenated solution on myocardial injury in rats with acute carbon monoxide poisoning

目的评价高氧液对急性一氧化碳（CO）中毒大鼠心肌损伤的影响。方法清洁级成年雄性SD大鼠30只，体重250—300g。采用随机数字表法分为5组（n=6）：对照组（C组）、急性CO中毒组（ACP组）和不同剂量高氧液组（HP1-3组）。采用腹腔注射CO120ml／kg的方法制备大鼠急性CO中毒模型，HP1-3组于腹腔注射CO后1h时分别尾静脉输注高氧液10、15和20ml／kg。于腹腔注射CO后24h时尾静脉取血样，应用全自动生化分析仪检测血浆肌酸激酶（CK）、肌酸激酶同工酶（CK—MB）、乳酸脱氢酶（LDH）和α-羟基丁酸脱氢酶（α-HBDH）的活性。随后处死大鼠取心肌组织，光镜下观察病理学结果。结果与c组比较，ACP组和HP1-3组血浆LDH、α—HBDH、CK和CK—MB活性升高（P〈0．01）；与ACP组比较，HP1-3组血浆LDH、α—HBDH、CK和CK—MB活性降低（P〈0．05或0．01）；与HP1组比较，HP2,3组血浆LDH、α-HBDH、CK和CK—MB活性降低（P〈0．05）。HP1-3组心肌病理学损伤较ACP组明显减轻。结论高氧液可减轻急性CO中毒大鼠的心肌损伤。

Objective To evaluate the effect of hyperoxygenated solution on myocardial injury in the rats with acute＇carbon monoxide （CO） poisoning. Methods Thirty pathogen-free adult male Sprague- Dawley rats, weighing 250-300 g, were randomly divided into 5 groups （n = 6 each） using a random number table： control group （C group） , acute CO poisoning group （ACP group） , and different doses of hyperoxygenated solution groups （HP1-3 groups）. CO 120 ml/kg was injected intraperitoneally to establish the model of acute CO poisoning. Hyperoxygenated solution 10, 15 and 20 ml/kg were infused via the caudal vein at 1 h after intraperitoneal injection of CO in HP1-3 groups, respectively. At 24 h after intraperitoneal injection of CO, blood samples were collected from the caudal vein for determination of plasma creatine kinase （CK）, ereatine kinase-MB （CK-MB）, lactic dehydrogenase （LDH） and alpha-hydroxybutyrate acid dehydrogenase （α-HBDH） activities using the automatic biochemical analyzer. The rats were then sacrificed, and myocardial specimens were obtained for examination of the pathological changes with a light microscope. Results Compared with group C, the plasma LDH, α-HBDH, CK and CK-MB activities were significantly increased in ACP and HP1-3 groups （P〈0.01）. Compared with group ACP, the plasma LDH, α-HBDH, CK and CK-MB activities were significantly decreased in HP1-3 groups （P〈0.05 or 0.01）. Compared with group HP1, the plasma LDH, α-HBDH, CK and CK-MB activities were significantly decreased in HP2,3 groups （P〈0.05）. The pathological changes of myocardium were significantly attenuated in HP1-3 groups as compared with group ACP. Conclusion Hyperoxygenated solution can attenuate myocardial injury in the rats with acute CO poisoning.