HMGB1和TLR4在食管鳞癌组织中的表达及临床意义

Clinical significance of expression of high mobility group protein B1 and Toll-like receptor 4 in esophageal squamous cell carcinoma

目的:探讨高迁移率族蛋白B1(high mobility group protein B1,HMGB1)和Toll样受体4(Toll-like receptor 4,TLR4)在人食管鳞状细胞癌(食管鳞癌)组织中的表达及其临床意义.方法:选择72例食管鳞癌标本,15例癌旁正常组织标本,采用En Vision免疫组织化学染色法检测HMGB1和TLR4在食管鳞癌组织及癌旁正常组织中的表达,并应用统计学方法对其表达与临床病理因素进行分析.结果:食管鳞癌组织中HMGB1、TLR4的表达显著高于正常组织(P<0.05),且与淋巴结转移及TNM分期相关(P<0.05),与肿瘤大小、分化程度等无相关性.食管麟癌组织HMGB1和TLR4的表达呈显著正相关(r=0.377,P<0.01).结论:食管鳞癌组织中HMGB1、TLR4的表达显著高于癌旁正常组织,且其表达与淋巴结转移及TNM分期相关,联合检测二者可能有助于评估食管鳞癌的恶性程度.因此,HMGB1/TLR信号通路有可能作为反映食管癌预后的重要生物学指标及抗食管癌的重要靶点.

AIM： To detect the expression of high mobility group protein B1 （HMGB1） and Toll-like receptor 4 （TLR4） in human esophageal squarnous cell carcinoma and analyze their clinical significance.METHODS： The expression of HMGB1 and TLR4 was detected by EnVision immunohisto- chemical staining method in 72 esophageal squamous carcinoma specimens and 15 matched normal tissue specimens. Statistical methods were used to analyze the relationship between the expression of HMGB1 and TLR4 and clinical and pathological parameters.RESULTS： The expression of HMGB1 and TLR4 in esophageal squamous carcinoma tissues was significantly higher than that in matched normal tissues （P 〈 0.05）. HMGB1 and TLR4 expression was positively associated with lymphatic metastasis and TNM stage （P 〈0.05）, but negatively correlated with tumor size and degree of differentiation. The expression of HMGB1 and TLR4 had a significant positive correlation （r = 0.377, P 〈 0.01）.CONCLUSION： The expression of HMGB1 and TLR4 in esophageal squamous carcinoma tissues is associated with lymphatic metastasis and TNM stage, and the joint detection of HMGB1 and TLR4 expression may help evaluate the degree of malignancy of esophageal squamous carcinoma. HMGB1/TLR may be used as important biological indicators reflecting the prognosis of esophageal cancer and important targets for therapy of esophageal cancer.