不同糖对拟南芥种子萌发和sscd1突变体细胞死亡的影响

Effects of Different Sugars on Arabidopsis Seed Germination and sscd1 Mutant Cell Death

[目的]比较不同糖对拟南芥种子萌发以及sscd1突变体细胞死亡的影响。[方法]以拟南芥Col-0野生型和sscd1突变体为材料,在培养基中外源添加蔗糖、葡萄糖、果糖和麦芽糖,比较不同糖对拟南芥种子萌发和sscd1突变体细胞死亡的影响。[结果]在120 mmol/L浓度范围内,蔗糖、葡萄糖和果糖对拟南芥种子的萌发无显著影响,而麦芽糖对种子萌发有延迟作用,且糖浓度越高延迟作用越显著。120 mmol/L不同糖对sscd1突变体细胞死亡的抑制效果有显著差异,抑制效果由高到低依次为麦芽糖、蔗糖、葡萄糖和果糖。[结论]该研究为深入研究糖代谢途径调控酪氨酸降解途径的机理提供理论依据。

Objective] To compare the effects of different sugars on Arabidopsis seed germination and sscd1 mutant cell death.[Method] With wide type （Col-0） and the mutant sscd1 as research materials, effects of different sugars on Arabidopsis seed germination and sscd1 cell death were compared by adding sucrose, glucose, fructose and maltose in the culture medium.[Result] At the 120 mol/L concentration range, su-crose, glucose and fructose had no significant effects on Arabidopsis seed germination, but maltose delayed seed germination.And high sugar con-centration had more significant delaying effects.Different sugars at 120 mol/L concentration showed significant differences in the inhibitory effects on sscd1 cell death, and the effects from high to low were in the order of maltose, sucrose, glucose and fructose.[Conclusion] This research pro-vides important foundation for the in-depth mechanism research on regulating of tyrosine degradation pathway by glucose metabolic pathway .