SYSMEX XE-5000全自动血液分析仪在儿童脑脊液常规检测中的应用

Application of SYSMEX XE-5000 automatic blood analyzer for the routine determination of cerebrospinal fluid among children

目的探讨SYSMEX XE-5000全自动血液分析仪(简称XE-5000)在儿童脑脊液常规检测中的应用价值。方法选取194份住院患儿的脑脊液标本,采用XE-5000体液模式进行脑脊液常规检测,同时行人工显微镜镜检,比较仪器法与人工镜检法检测结果的符合性。结果当白细胞(WBC)计数≥30个/μL时,2种方法差异无统计学意义(P>0.05)。当WBC计数<30个/μL时,2种方法差异有统计学意义(P<0.05)。当WBC计数≥10个/μL时,2种方法之间单个核细胞计数及多个核细胞计数均具有良好的相关性(P<0.01)。当红细胞(RBC)计数>1 000个/μL时,2种方法具有良好的相关性(r=0.98,P<0.01)。结论应用XE-5000体液模式检测儿童脑脊液,在WBC总数≥30个/μL时具有较好的应用价值,可代替人工镜检。

ObjectiveTo investigate the signiflcance of SYSMEX XE-5000 automatic blood analyzer for the routine determination of cerebrospinal fluid among children. MethodsA total of 194 cerebrospinal fluid samples were collected and determined by XE-5000 and manual microscopy. The consistency was evaluated. ResultsWhen white blood cell（WBC） count was ≥30 cells/μL,there was no statistical signiflcance between the 2 methods（P〉0.05）. However,when WBC count was 〈30 cells/μL,there was a statistical signiflcance between the 2 methods（P〈0.05）. When WBC count was ≥10 cells/μL,there were good correlations in counting mononuclear cells and multinuclear cells between the 2 methods （P〈0.01）. When red blood cell（RBC） count was 〉1 000 cells/μL,there was a good correlation between the 2 methods（r=0.98,P〈0.01）. ConclusionsXE-5000 can be used in the determination of cerebrospinal fluid among children,when WBC count is ≥30 cells/μL.