茶多糖对阿霉素抑制肺癌A549细胞增殖作用的影响

Tea Polysaccharide Increased Doxorubicin Inhibition of Lung Cancer A549 Cells

肺癌是全世界目前发病率和死亡率最高的癌症之一,当前治疗恶性肿瘤的主要手段之一是化疗,阿霉素是临床常用的化疗药物,然而该药物毒性较大,长期使用可发生剂量依赖性的不可逆的心肌病变、骨髓抑制等,同时其多药耐药性的存在也使它在临床应用受到一定限制。为了减少阿霉素的毒副作用,通过体外培养人肺癌A549细胞,将茶叶提取物茶多糖与阿霉素联用,加入A549细胞,24 h后以噻唑蓝(MTT)法检测细胞存活率。结果表明,当阿霉素质量浓度为3 mg·L^(-1)时,对肺癌A549细胞的抑制效果最明显;不同浓度茶多糖与1、2、3 mg·L^(-1)阿霉素联用,以2 mg·L^(-1)阿霉素与6 mg·L^(-1)茶多糖联用时对肺癌A549细胞的抑制效果最明显,且优于单独使用3 mg·L^(-1)阿霉素的效果。阿霉素可诱导A549细胞凋亡,茶多糖与阿霉素联用可减少阿霉素的使用剂量,增强阿霉素对肺癌A549细胞的增殖抑制作用。

Lung cancer has high incidence and mortality rates around the world and chemotherapy is widely applied in cancer treatment. Doxorubicin （DOX） is a kind of chemotherapy drug which commonly used in clinical trials. However, the drug has many side effects and may cause a dose-dependent injury in the long term. These side effects include irreversible cardiopulmonary, bone marrow suppression and so on. Moreover, its mufti-drug resistance also restricts it in certain clinical application. The effect of polysaccharides of tea extract combined with doxorubicin on human lung cancer cell line A549 were tested in this study. A549 was first cultured in vitro with different concentrations of DOX. After 24h culture, apotheosis ratio was determined by MTT. The results show that the DOX dose for the best inhibiting effect was 3 mg·L^-1 according to MTT detection. Three doses of DOX were selected to combine tea polysaccharide and suppress A549 cell survival. The combination of 2 mg·L^-1 DOX and 6 mg·L^-1 tea polysaccharide had been demonstrated to have better suppressing efficiency than 3 mg·L^-1 DOX alone. DOX could efficiently suppress A549 cell proliferation and this inhibiting effect was enhanced by combining with tea polysaccharide, which could decrease the applied dose of DOX.